Get the free -Fixed Paraffin Embedded Tissue FFPE FISH Request Form

FormalinFixed Paraffin Embedded Tissue (FCPE) FISH Request Form Please note: All paraffin section FISH cases should be accompanied by an H & E slide

How to fill out fixed paraffin embedded tissue

How to fill out fixed paraffin embedded tissue:

1. Collect the tissue sample: Start by collecting the tissue sample of interest, ensuring it is representative of the area you want to study. This can be done through various methods, such as biopsies or surgical procedures.
2. Fixation: Once the tissue sample has been obtained, it needs to be fixed to preserve its morphology and prevent degradation. The most common fixative used is formalin, which should be applied for an appropriate amount of time (usually 24-48 hours) to ensure proper fixation.
3. Dehydration: After fixation, the tissue needs to go through a series of dehydrating agents (such as ethanol) to remove all water content. This process is usually carried out in a series of increasing ethanol concentrations.
4. Clearing: Following dehydration, the tissue needs to be cleared using a clearing agent, such as xylene or toluene. This step helps to remove the dehydrating agent and allows the paraffin to penetrate the tissue.
5. Infiltration: Once the tissue has been cleared, it is ready for infiltration with molten paraffin wax. The tissue should be placed in a container with the molten paraffin and left to infiltrate for an appropriate amount of time.
6. Embedding: After infiltration, the tissue is ready for embedding. Transfer the tissue into a mold, ensuring it is properly oriented, and pour molten paraffin wax around the tissue. Allow the wax to solidify.
7. Block trimming: Once the paraffin has hardened, the block needs to be trimmed to expose the tissue surface. This can be done using a microtome or a similar cutting instrument.
8. Sectioning: With the block trimmed, it is ready for sectioning. Thin sections (usually around 4-6 micrometers) are cut from the block using a microtome. These sections are then mounted onto glass slides.
9. Staining and analysis: The mounted sections can now undergo staining and analysis, such as hematoxylin and eosin (H&E) staining, immunohistochemistry, or other specific techniques, depending on the research or diagnostic needs.

Who needs fixed paraffin embedded tissue?

1. Histologists: Histologists require fixed paraffin embedded tissue for routine microscopic examination and diagnosis of diseases.
2. Researchers: Researchers in various scientific fields, such as biology, medicine, or pathology, often use fixed paraffin embedded tissue for their studies, including molecular analysis, gene expression profiling, and immunohistochemistry.
3. Pathologists: Pathologists use fixed paraffin embedded tissue to establish diagnoses, determine the extent of diseases, or monitor the effectiveness of treatments in patients.
4. Biobanks: Biobanks store fixed paraffin embedded tissue as a resource for future research or clinical investigations.
5. Geneticists: Geneticists may utilize fixed paraffin embedded tissue to analyze DNA or RNA content, study genetic mutations, or investigate gene expression patterns.

Overall, fixed paraffin embedded tissue is relevant to histology, research, pathology, biobanking, and genetic analysis, serving various purposes in both diagnostic and scientific fields.

For pdfFiller’s FAQs

What is fixed paraffin embedded tissue?

Fixed paraffin embedded tissue is tissue that has been treated with chemicals to preserve its structure and is embedded in paraffin wax for storage and analysis.

Who is required to file fixed paraffin embedded tissue?

Medical professionals and researchers who use fixed paraffin embedded tissue for studies or diagnostic purposes are required to file it.

How to fill out fixed paraffin embedded tissue?

Fixed paraffin embedded tissue is typically filled out by providing information about the specimen, the processing methods used, and any relevant findings.

What is the purpose of fixed paraffin embedded tissue?

Fixed paraffin embedded tissue is used for histological analysis, research studies, and diagnostic testing in medical settings.

What information must be reported on fixed paraffin embedded tissue?

Information such as patient identification, specimen type, processing methods, and any relevant findings must be reported on fixed paraffin embedded tissue.

How can I send fixed paraffin embedded tissue to be eSigned by others?

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