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UNIT 24: Coliform Bacteria and Water Quality Lab AP Environmental Science, Mr. Doc Miller North Central High School Name: Period: Lab Team: Date: LAB: Coliform Bacteria and Water Quality Introduction:
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How to fill out lab coliform bacteria and:

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Gather necessary equipment: Before starting, make sure you have all the required equipment, including petri dishes, agar medium, pipettes, sterilized water, and a Bunsen burner for sterilization.
02
Prepare the agar medium: Follow the instructions provided to prepare the agar medium. This may involve heating the agar solution, pouring it into petri dishes, and allowing it to solidify.
03
Incubation: Take the prepared petri dishes and inoculate them with a small amount of the sample being tested. Make sure to spread the sample evenly across the agar surface using a sterilized loop or pipette.
04
Label and seal: Once the samples are inoculated, label each petri dish with essential information such as the date, sample source, and any other relevant details. Seal the plates using paraffin film or adhesive tape to prevent contamination.
05
Incubate the plates: Place the sealed petri dishes in an incubator set at the appropriate temperature for coliform bacteria growth, usually around 37°C. Allow them to incubate for the specified period, typically 24-48 hours.
06
Interpretation of results: After incubation, carefully examine the petri dishes for any visible signs of coliform bacterial growth. Look for characteristic colonies or zones of color change according to the specific testing method used.

Who needs lab coliform bacteria and:

01
Food industries: Lab coliform bacteria testing is crucial in the food industry to ensure safety and quality control. It helps identify potential contamination sources and prevents the distribution of products with harmful bacteria.
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Environmental agencies: Monitoring water quality is a vital task performed by environmental agencies. Lab coliform bacteria testing helps identify possible water pollution sources, assess the effectiveness of wastewater treatment, and protect public health.
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Healthcare facilities: Hospitals, clinics, and other healthcare settings need to test for coliform bacteria to maintain a safe environment for patients and staff. Regular testing helps identify potential sources of nosocomial infections and implement appropriate infection control measures.
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Lab coliform bacteria testing is a method used to measure bacterial contamination in water.
Public water systems and certain private water systems are required to file lab coliform bacteria testing results.
Lab coliform bacteria testing can be conducted by collecting water samples and sending them to a certified laboratory for analysis.
The purpose of lab coliform bacteria testing is to ensure that drinking water is safe and free from harmful bacteria.
The lab coliform bacteria testing report must include details on the water source, sampling location, date and time of collection, and test results.
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