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DIAGNOSTIC NEOPLASTIC CYTOGENETICS SPECIMEN INCORPORATED REQUEST 1525 13th Ave. Seattle, WA 98122 1525 13th Ave. Seattle, WA 98122 8003282026 fax 2063252975 8003282026 faxes 2063252975 www.diagnosticcytogenetics.com

How to fill out paraffin embedded tissue type

How to Fill Out Paraffin Embedded Tissue Type:

1. Prepare the tissue sample: Start by obtaining the tissue sample that needs to be embedded in paraffin. Ensure that the sample is appropriately preserved and free from any contaminants.
2. Process the tissue: Begin by fixing the tissue sample in a suitable fixative solution for a specified period. This step helps in preserving the cellular structure and preventing degradation.
3. Dehydrate the tissue: After fixing, dehydrate the tissue sample using a series of alcohol solutions with increasing concentration. This process removes the water from the sample to make it compatible with the paraffin embedding process.
4. Clearing agents: The tissue sample needs to be treated with a clearing agent, such as xylene or toluene, to remove alcohol residues and ensure optimal penetration of the paraffin wax.
5. Infiltrate with paraffin wax: Place the tissue sample in a container with liquid paraffin wax and keep it at an appropriate temperature. The wax will gradually infiltrate the sample, replacing the clearing agent, and ensuring proper embedding.
6. Embed the tissue: Once the tissue has been adequately infiltrated, carefully transfer it into a suitable mold containing liquid paraffin wax. Ensure that the tissue is oriented correctly and securely positioned within the mold.
7. Solidify the paraffin: Allow the paraffin wax to cool and solidify, trapping the tissue sample within it. This step ensures that the tissue is encased in a firm and stable medium, facilitating subsequent sectioning.
8. Section the tissue: Use a microtome to cut thin slices from the paraffin-embedded tissue block. These sections are typically placed on glass slides for further analysis or staining.
9. Mount the sections: Transfer the tissue sections onto glass slides, ensuring proper orientation. To facilitate better adhesion, the slides can be coated with an adhesive such as poly-L-lysine.
10. Store the slides: Once the sections are mounted, store the slides in a suitable slide box or other storage containers, protecting them from potential damage or contamination.

Who needs paraffin embedded tissue type?

1. Pathologists: Paraffin embedding allows pathologists to prepare tissue samples for microscopic examination, aiding in the diagnosis of various diseases and conditions.
2. Research Scientists: Paraffin-embedded tissue sections serve as a valuable resource for research studies, enabling the investigation of cellular structures, disease mechanisms, and therapeutic targets.
3. Biobanks: Paraffin-embedded tissue samples are commonly stored in biobanks, as these preserved specimens provide a rich source of biological material for future research and potential clinical applications.
4. Medical Laboratories: Medical laboratories often utilize paraffin-embedded tissue sections for various diagnostic tests, including immunohistochemistry, molecular analysis, and histopathological evaluations.

Overall, paraffin embedded tissue type is essential for individuals involved in pathology, research, biobanking, and medical testing to enable accurate diagnosis, research advancements, and improved patient care.

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What is paraffin embedded tissue type?

Paraffin embedded tissue type refers to tissues that have been preserved in paraffin wax for analysis or storage purposes.

Who is required to file paraffin embedded tissue type?

Medical professionals, researchers, and laboratories may be required to file paraffin embedded tissue type depending on the specific regulations or guidelines.

How to fill out paraffin embedded tissue type?

To fill out paraffin embedded tissue type, one must provide relevant information about the tissue sample, including the patient's details, the type of tissue, and any specific observations or findings.

What is the purpose of paraffin embedded tissue type?

The purpose of paraffin embedded tissue type is to preserve tissue samples for future analysis, research, or diagnostic purposes.

What information must be reported on paraffin embedded tissue type?

Information such as patient details, tissue type, date of collection, processing methods, and any relevant observations must be reported on paraffin embedded tissue type.