Get the free Lymphoblastoid cell lines - lcl

Center for Cell & Gene Therapy CENTER FOR CELL & GENE THERAPY GMP FACILITY, 16th FLOOR, FEIGN CENTER 1102 BATES STREET, HOUSTON, TEXAS 77030 CAST M P Good Manufacturing Practices Facility SOP: D03.01.23

How to fill out lymphoblastoid cell lines

How to fill out lymphoblastoid cell lines:

Start by preparing the culture medium:

1. Combine the appropriate amount of growth medium with necessary supplements such as serum.
2. Adjust the pH and sterilize the medium using filtration or autoclaving.

Thaw the frozen lymphoblastoid cell line:

1. Retrieve the vial(s) containing the frozen cells from liquid nitrogen.
2. Quickly transfer the vial(s) to a 37°C water bath until the contents have completely thawed.
3. Gently agitate the vial(s) to ensure homogeneous thawing.

Seed the cells in a culture flask:

1. Sterilize the flask by treating it with a disinfectant and rinsing with sterile water.
2. Calculate the appropriate cell density for seeding, usually based on the desired confluency or experimental requirements.
3. Transfer the thawed cells into the flask containing the prepared culture medium.
4. Incubate the flask at 37°C with appropriate gas mixture (e.g., 5% CO2) and humidity.

Monitor cell growth and maintain the culture:

1. Regularly check the cell culture for signs of contamination, pH changes, or excessive cell density.
2. Adjust the culture medium as necessary by either adding fresh medium or changing it completely.
3. Perform regular cell counts or viability assessments to determine cell doubling time and overall culture health.

Who needs lymphoblastoid cell lines:

1. Researchers working in immunology: Lymphoblastoid cell lines are valuable tools for studying immune responses, vaccine development, and infectious diseases.
2. Pharmaceutical companies: Lymphoblastoid cell lines can be used to test the efficacy and safety of drugs that target the immune system or interact with lymphocytes.
3. Geneticists and genomic researchers: Lymphoblastoid cell lines are utilized in genetic studies, including genome-wide association studies (GWAS) and investigating hereditary diseases.
4. Biotechnology companies: Lymphoblastoid cell lines are employed in the production of therapeutic proteins, monoclonal antibodies, and immunotherapies.

In summary, understanding how to fill out lymphoblastoid cell lines involves preparing the culture medium, thawing frozen cells, seeding them in a culture flask, and maintaining the culture conditions. These cell lines are utilized by researchers in immunology, pharmaceutical companies, geneticists, and biotechnology companies for various applications.

For pdfFiller’s FAQs

What is lymphoblastoid cell lines?

Lymphoblastoid cell lines are immortalized cell lines that are commonly used in research, particularly in studies involving the immune system.

Who is required to file lymphoblastoid cell lines?

Researchers and laboratories working with lymphoblastoid cell lines are required to file them.

How to fill out lymphoblastoid cell lines?

Lymphoblastoid cell lines are typically filled out by providing information about the source of the cells, the culture conditions, and any relevant experimental protocols.

What is the purpose of lymphoblastoid cell lines?

The purpose of lymphoblastoid cell lines is to serve as a model system for studying various biological processes and disease mechanisms.

What information must be reported on lymphoblastoid cell lines?

Information such as the origin of the cells, passage number, cell growth conditions, and any relevant experimental treatments must be reported on lymphoblastoid cell lines.

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