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Li et al. BMC Genomics (2015) 16:1069 DOI 10.1186/s1286401522701 RESEARCH ARTICLE Open Access Comprehensive evaluation of Applied transcriptome, a novel targeted whole transcriptome RNA sequencing
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Start by carefully reviewing the instructions provided in the comprehensive evaluation of AmpliSeq document. Ensure you understand all the requirements and steps involved.
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Begin by gathering all the necessary materials and reagents mentioned in the instructions. This may include the AmpliSeq panel, library preparation kit, sequencing platform, and any other specified components.
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Prepare the samples according to the instructions provided. This may involve extracting DNA or RNA from the desired source material and quantifying the amount of nucleic acid obtained.
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Follow the specified protocols for library preparation. This typically involves performing PCR amplification, purification, and quantification steps using the AmpliSeq panel and library preparation kit.
05
After the libraries are prepared, perform quality control measures such as assessing library size, concentration, and fragment distribution. Use appropriate analytical instruments or software for this purpose.
06
Follow the instructions for sequencing the libraries using the designated sequencing platform. Ensure that the correct sequencing parameters are set according to the requirements provided.
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Finally, upon completion of the sequencing run, analyze the sequencing data using appropriate bioinformatics tools or software. This may involve mapping the reads, variant calling, and data interpretation.

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Pharmaceutical companies or biotechnology firms developing targeted therapies or precision medicine approaches, as comprehensive evaluation of AmpliSeq can provide valuable insights into the genetic profile of patient samples or model systems.
In summary, individuals who aim to explore genetic variations within specific genomic regions or require detailed knowledge about the targeted regions of interest can benefit from performing a comprehensive evaluation of AmpliSeq.
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