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10444 EHOS USE ONLY PROTOCOL FOR ANIMAL USE AND CARE Email to: campus vet McDavis.edu PROTOCOL: EXPIRES: CNPC Investigator 10444 1/16/04 Contact Last Name: Last Name: First: First: Middle: Middle:

How to fill out macaque sperm cryopreservation

How to fill out macaque sperm cryopreservation?

1. Collect the semen: Use a suitable collection method to obtain the macaque sperm. This can be done through electroejaculation or other non-invasive methods.
2. Assess semen quality: Evaluate the semen sample for motility, concentration, and other parameters, using techniques such as computer-assisted sperm analysis (CASA).
3. Prepare cryoprotectant solution: Create a cryoprotectant solution that will help protect the sperm during freezing and storage. This solution typically contains a combination of extenders, antioxidants, and cryoprotectants like glycerol or dimethyl sulfoxide (DMSO).
4. Dilute the semen: Mix the collected semen with the cryoprotectant solution to achieve the desired sperm concentration. This step is crucial for maintaining sperm viability during freezing.
5. Packaging and labeling: Transfer the diluted sperm into appropriate cryovials or straws, ensuring proper labeling with necessary information like sample ID, date, and donor details.
6. Cooling process: Place the packaged samples in a controlled-rate cooling machine or programmable freezer. Gradually lower the temperature to -80°C or lower, following a specific cooling protocol to prevent damage to the sperm.
7. Storage: Transfer the sealed cryovials or straws to liquid nitrogen for long-term storage. Maintain the samples at -196°C to ensure the preservation of sperm viability.
8. Record keeping: Maintain detailed records of the entire cryopreservation process, including collection details, assessment results, cryoprotectant formulation, cooling rates, and storage information.

Who needs macaque sperm cryopreservation?

1. Researchers and scientists studying primate reproduction: Macaque sperm cryopreservation is commonly utilized in scientific research to study primate reproductive biology, assisted reproductive techniques, and the effects of various treatments or interventions on sperm.
2. Conservation and breeding programs: Cryopreservation of macaque sperm is crucial for preserving genetic diversity and maintaining viable populations in conservation and breeding programs. This technique allows the storage and transport of genetic material between different facilities, ensuring sustainable management of these primate species.
3. Assisted reproductive technology clinics: Macaque sperm cryopreservation is also valuable in providing reproductive solutions for non-human primates. It enables the availability of cryopreserved sperm for artificial insemination, in vitro fertilization (IVF), or intracytoplasmic sperm injection (ICSI) procedures, facilitating assisted reproduction in macaque species.

In summary, the process of filling out macaque sperm cryopreservation involves collecting, evaluating, diluting, packaging, cooling, and storing the semen using cryoprotectant solutions. This technique is valuable for a diverse range of professionals, including researchers, conservationists, and clinicians involved in primate reproduction and assisted reproductive technology.

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What is macaque sperm cryopreservation?

Macaque sperm cryopreservation is the process of freezing and storing sperm from macaque monkeys for future use.

Who is required to file macaque sperm cryopreservation?

Researchers or institutions working with macaques are required to file for macaque sperm cryopreservation.

How to fill out macaque sperm cryopreservation?

To fill out macaque sperm cryopreservation, one needs to follow the guidelines provided by the relevant regulatory body and submit all the required information accurately.

What is the purpose of macaque sperm cryopreservation?

The purpose of macaque sperm cryopreservation is to preserve genetic material from macaques for research, breeding, or conservation purposes.

What information must be reported on macaque sperm cryopreservation?

The information reported on macaque sperm cryopreservation may include details of the macaque donor, collection date, processing method, and storage conditions.