Get the free S100A9 (Human) ELISA Kit Cat

S100A9 (Human) ELISA Kit Cat. No.:DEIA4162 Pkg. Size:96T Intended use This kit is used for the quantitative measurement of human S100A9/MRP14 in serum, plasma and other biological media. General Description

How to fill out s100a9 human elisa kit

How to fill out s100a9 human ELISA kit:

1. Ensure you have read and understood the instructions provided with the kit. Familiarize yourself with the components included in the kit, such as microplates, standards, controls, and reagents.
2. Prepare all necessary materials and reagents in a clean and sterile working area. This may include distilled water, pipettes, plates, plate sealers, and a plate reader for measuring absorbance.
3. Thaw all frozen components of the kit, if any, according to the instructions. Ensure that all components are at room temperature before use.
4. Label the microplate wells according to your experimental design or as instructed in the kit manual. Make sure to follow the correct arrangement for standards, controls, and samples.
5. Using a multichannel pipette or a repeating pipette, add the standards, controls, and samples into the respective wells. Be careful not to cross-contaminate or introduce air bubbles.
6. Add the enzyme-conjugate reagent (provided in the kit) to each well. This reagent will bind to any target analytes present in the samples, forming a sandwich complex.
7. Incubate the plate at the recommended temperature and duration. This allows sufficient time for the reaction to occur and the sandwich complexes to form.
8. After the incubation period, wash the plate thoroughly with the provided wash buffer. This step removes any unbound materials and reduces background noise.
9. Add the substrate solution to each well. The enzyme-conjugate bound to the target analyte will catalyze a reaction, resulting in a color change.
10. Incubate the plate again for a specified period, allowing the substrate reaction to develop. The color intensity will be directly proportional to the amount of target analyte in the sample.
11. Stop the reaction by adding the stop solution. This will halt the enzymatic activity and fix the color at its current intensity.
12. Use a plate reader to measure the absorbance of each well at the appropriate wavelength. Compare this absorbance with the provided standard curve to estimate the concentration of the target analyte in each sample.

Who needs s100a9 human ELISA kit?

1. Researchers studying the role of s100a9 protein in human biology to gain insights into its functions and interactions with other molecules.
2. Clinicians and medical professionals interested in monitoring s100a9 levels in patient samples for diagnostic or prognostic purposes. Elevated levels of s100a9 have been associated with various diseases, including inflammation, cancer, and autoimmune disorders.
3. Pharmaceutical companies and drug development researchers involved in identifying potential therapeutic targets or evaluating the efficacy of new drugs. The s100a9 ELISA kit can be used to measure the concentration of s100a9 in biological samples before and after treatment, providing valuable information on drug response.
4. Academic institutions and biotechnology companies engaged in biomarker discovery projects. The s100a9 ELISA kit can be used to screen large cohorts of samples to identify potential biomarkers associated with certain conditions or disease progression.

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