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How to fill out truseq custom amplicon library
How to fill out truseq custom amplicon library:
01
Prepare DNA samples: Extract DNA from your desired sample using a DNA extraction kit. Ensure that the DNA concentration is accurately measured.
02
Design custom amplicon panel: Utilize the TruSeq Custom Amplicon Designer online tool to design a custom panel based on your specific targets of interest. Select the desired amplicon length, primer design strategy, and enter the target regions.
03
Order custom panel: Once you have finalized the design, place an order for the custom panel with an authorized supplier.
04
Prepare libraries: Allocate 50 ng of each DNA sample, and synthesize the libraries using the TruSeq Custom Amplicon Index Kit. Follow the manufacturer's protocol for library preparation, ensuring accurate mixing and handling.
05
Amplify libraries: Utilize the Illumina TruSeq Custom Amplicon PCR Master Mix to amplify the libraries. Follow the manufacturer's instructions for thermal cycling conditions and reaction setup.
06
Validate libraries: Analyze the quality and quantity of the libraries using a Qubit Fluorometer or agarose gel electrophoresis. Accurate quantification is crucial for pooling equal amounts of each library.
07
Pool libraries: Combine an equal amount of each library into a single pooled library, ensuring a total DNA concentration suitable for subsequent sequencing.
08
Perform sequencing: Submit the pooled library to an Illumina-approved sequencing service provider. Determine the appropriate sequencing platform and read length based on the expected coverage and target depth.
09
Analyze data: Once sequencing is complete, obtain the raw sequence reads and perform bioinformatics analysis to align the reads to the reference genome, identify variants, and interpret the results.
Who needs truseq custom amplicon library:
01
Researchers working in genomics: TruSeq custom amplicon library is beneficial for researchers in genomics who aim to study specific target regions in the genome with high sensitivity and accuracy.
02
Clinical laboratories: TruSeq custom amplicon library can be utilized by clinical laboratories for diagnostics and screening purposes, enabling the identification of genetic variations or mutations in specific genes associated with diseases.
03
Agricultural scientists: TruSeq custom amplicon library can be valuable for agricultural scientists studying plant or animal genomes, allowing them to assess specific genetic markers or traits of interest.
Note: The information provided above is for illustrative purposes only. It is essential to refer to the official protocols, guidelines, and instructions provided by Illumina or authorized suppliers for accurate and up-to-date information on the TruSeq Custom Amplicon Library workflow.
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What is truseq custom amplicon library?
TruSeq Custom Amplicon Library is a product that enables targeted amplification and sequencing of specific regions of interest in the genome.
Who is required to file truseq custom amplicon library?
Researchers and laboratories performing targeted sequencing experiments may be required to use TruSeq Custom Amplicon Library kits.
How to fill out truseq custom amplicon library?
To fill out the TruSeq Custom Amplicon Library, users need to follow the protocol provided by the manufacturer, which includes library preparation, target enrichment, and sequencing steps.
What is the purpose of truseq custom amplicon library?
The purpose of TruSeq Custom Amplicon Library is to enable researchers to focus on specific genomic regions of interest, allowing for targeted sequencing and analysis.
What information must be reported on truseq custom amplicon library?
Users may need to report details such as the target regions of interest, the number of samples, sequencing parameters, and any modifications to the protocol used.
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