Get the free REDExtract-N-Amp Tissue PCR Kit

REDExtractNAmp Tissue PCR Kit Catalog Numbers XNAT10RXN, XNAT100RXN, XNAT1000RXNTECHNICAL BULLETIN Product Description The REDExtractNAmp Tissue PCR Kit contains all the reagents needed to rapidly

How to fill out redextract-n-amp tissue pcr kit

How to Fill Out Redextract-n-Amp Tissue PCR Kit:

1. Start by gathering all the necessary materials for the Redextract-n-Amp Tissue PCR Kit. This includes the kit itself, a tissue sample, microcentrifuge tubes, pipettes, and a thermal cycler.
2. Begin the process by selecting a tissue sample that you wish to extract DNA from. The Redextract-n-Amp Tissue PCR Kit is designed specifically for efficient DNA extraction from various animal tissues, including mouse tails, ear punches, and fin clips.
3. Carefully cut a small piece of the tissue sample using sterile tools. Ensure that the sample is small enough to fit into a microcentrifuge tube, but large enough to provide sufficient DNA for downstream PCR applications.
4. Add the tissue sample to a microcentrifuge tube containing the lysis solution provided in the kit. Make sure to label the tube appropriately to avoid any confusion later on. The lysis solution acts on the tissue, breaking down the cellular membranes and releasing the DNA.
5. Mix the tissue and lysis solution by gently vortexing or pipetting up and down several times. It is important to ensure thorough mixing to facilitate effective lysis and DNA extraction.
6. After mixing, the microcentrifuge tube should be incubated at a specific temperature for a designated period of time. The kit instructions will provide the recommended incubation temperature and duration. This step allows the lysis solution to effectively extract the DNA from the tissue sample.
7. Once the incubation period is complete, the microcentrifuge tube is ready to be heated. This step is known as the heat inactivation step and it serves to inactivate any enzymes present in the lysis solution that may interfere with subsequent PCR reactions.
8. Next, the heated sample is cooled and can be directly used as a DNA template for PCR amplification. Transfer an aliquot of the solution to a new microcentrifuge tube for this purpose. The Redextract-n-Amp Tissue PCR Kit is specifically designed for compatibility with the subsequent PCR steps, making it convenient for amplifying extracted DNA without the need for additional purification steps.

Who needs Redextract-n-Amp Tissue PCR Kit?

1. Researchers or scientists working in molecular biology or genetics laboratories who require a quick and efficient method for DNA extraction from animal tissues.
2. Individuals performing genetic analysis, such as genotyping or genetic fingerprinting, and require a reliable and user-friendly DNA extraction kit.
3. Laboratories or clinics involved in diagnostic procedures that require the extraction of DNA from animal tissues for identifying or detecting specific genetic markers or mutations.

Please note that the specific needs may vary, and it is always recommended to consult the product instructions or seek guidance from experts in order to ensure the proper and effective use of the Redextract-n-Amp Tissue PCR Kit.

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