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REQUEST FOR PROPOSALS FOR PHYTOPLANKTON AND ZOOPLANKTON ENUMERATION SERVICES SACRAMENTO REGIONAL COUNTY SANITATION DISTRICT POLICY AND PLANNING DEPARTMENT ISSUE DATE: April 20, 2016, DUE DATE: May
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How to fill out phytoplankton and zooplankton enumeration

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How to fill out phytoplankton and zooplankton enumeration?

01
Start by preparing the necessary equipment and materials. You will need a microscope, slides, cover slips, a plankton net or sampler, a sampling container, and preservatives such as formalin or Lugol's solution.
02
Decide on the sampling method that best suits your study objectives. Common methods include vertical tows, horizontal tows, bottle sampling, and sediment sampling. Each method has its own specific instructions, so be sure to follow the appropriate protocol.
03
Once you have collected the water sample, transfer a small volume (usually around 100 mL) to a measuring cylinder. Record the volume of the sample for later calculations.
04
Add a few drops of the appropriate preservative (formalin or Lugol's solution) to the water sample. This will help preserve the plankton and prevent decomposition.
05
Mix the sample thoroughly to ensure even distribution of the preservative. Allow the sample to settle for a few minutes.
06
Take a small aliquot (a representative subsample) from the settled sample using a pipette. Place this subsample onto a clean microscope slide.
07
Carefully place a cover slip over the subsample on the slide, taking care to avoid trapping any air bubbles. Gently press down on the cover slip to flatten the subsample.
08
Using a microscope, examine the subsample under low magnification first to locate and identify any larger organisms. Then, switch to higher magnification to observe and enumerate the phytoplankton and zooplankton.
09
Count and identify the organisms present in the subsample. This can be done by systematically scanning the slide from left to right and top to bottom, counting each individual organism within a predetermined field of view.
10
Repeat steps 6-9 for multiple subsamples, depending on the desired level of accuracy and statistical significance. The number of subsamples may vary depending on the study design and objectives.

Who needs phytoplankton and zooplankton enumeration?

01
Researchers and scientists studying marine or freshwater ecosystems often need to conduct phytoplankton and zooplankton enumeration. These organisms play crucial roles in ecological processes and can provide valuable insights into water quality, trophic dynamics, and overall ecosystem health.
02
Environmental monitoring agencies and regulatory bodies may require phytoplankton and zooplankton enumeration as part of their routine assessments. Monitoring changes in plankton communities can help detect and track environmental shifts, pollution events, or the presence of harmful algal blooms.
03
Educational institutions and teachers may incorporate phytoplankton and zooplankton enumeration into their curriculum to teach students about aquatic ecosystems, biodiversity, and ecological interactions. It can be a hands-on activity that enhances understanding of biological concepts and scientific methods.
Overall, anyone involved in ecological research, environmental monitoring, or education related to aquatic ecosystems can benefit from phytoplankton and zooplankton enumeration.
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Phytoplankton and zooplankton enumeration is the process of counting and identifying microscopic plant and animal organisms in bodies of water.
Researchers, environmental agencies, and organizations involved in water quality monitoring are typically required to file phytoplankton and zooplankton enumeration.
Phytoplankton and zooplankton enumeration forms are usually filled out by collecting samples of water, analyzing them under a microscope, and recording the findings.
The main purpose of phytoplankton and zooplankton enumeration is to assess the health of aquatic ecosystems and monitor changes in water quality.
Reports typically include the identification and count of phytoplankton and zooplankton species, as well as the location and date of the sampling.
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