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Quantizing ELISA Human IFN Immunoassay Catalog Number DIF50 Catalog Number SIF50 Catalog Number PDIF50 For the quantitative determination of human gamma (IFN) concentrations in cell culture superstates,
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How to fill out human ifn- quantikine
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Step 1: Begin by gathering all the necessary materials for the human IFN-Quantikine assay, which includes the kit, samples, reagents, and equipment.
02
Step 2: Prepare the samples by diluting them according to the instructions provided in the kit. Ensure that the dilutions are accurate and appropriate for the assay.
03
Step 3: Set up the assay plate by adding the provided standards, controls, and diluted samples to their respective wells. Follow the instructions carefully to avoid any errors.
04
Step 4: Incubate the assay plate at the recommended temperature and for the specified duration mentioned in the kit instructions.
05
Step 5: After the incubation period, wash the plate thoroughly to remove any unbound substances. Follow the washing steps provided in the kit.
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Step 6: Add the detection reagent to each well and incubate according to the kit instructions.
07
Step 7: After the incubation, wash the plate again to remove any unbound detection reagent.
08
Step 8: Add the substrate solution to each well and allow the color to develop.
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Step 9: Stop the reaction by adding the stop solution.
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Step 10: Measure the absorbance of each well using a microplate reader.
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Step 11: Calculate the concentration of human IFN by comparing the absorbance values of the standards and samples using the provided formula or software.
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Step 12: Record and analyze the results obtained from the assay, and interpret the concentration values accordingly.
Who needs human ifn- quantikine?
01
Researchers and scientists studying the immune response in humans.
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Clinicians and healthcare professionals monitoring the levels of human IFN-γ in patient samples.
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Pharmaceutical companies developing drugs or therapies that target the immune system.
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Laboratories performing diagnostic tests for infectious diseases or autoimmune disorders.
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Academic institutions conducting research on cytokines and their role in various biological processes.
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