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Immunohistochemistry and Special Stain Requisition Client Information Phone 866.776.5907/ Fax 239.690.4237 neogenomics.com Patient Information Last Name: First Name: M.I. Male Female Date of Birth:
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01
Step 1: Prepare the slides by fixing the tissue sections on them.
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Step 2: Deparaffinize the slides by soaking them in xylene or a xylene substitute.
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Step 3: Rehydrate the slides by soaking them in a series of decreasing concentrations of alcohol.
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Step 4: Prepare the antigen retrieval solution according to the specific antibody manufacturer's instructions.
05
Step 5: Heat the slides in the antigen retrieval solution using a microwave or a water bath.
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Step 6: Block the non-specific antibody binding sites by incubating the slides in a blocking solution.
07
Step 7: Incubate the slides with the primary antibody specific to the target antigen.
08
Step 8: Wash the slides to remove any unbound primary antibody.
09
Step 9: Incubate the slides with a secondary antibody conjugated with a detection system, such as HRP or a fluorescent dye.
10
Step 10: Wash the slides again to remove any unbound secondary antibody.
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Step 11: Apply the chromogen substrate or fluorochrome to visualize the antigen-antibody complex.
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Step 12: Counterstain the slides if desired.
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Step 13: Mount the slides with a coverslip using a mounting medium.
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Step 14: Allow the slides to dry and then examine them under a microscope for desired staining.

Who needs ihc amp special stain?

01
Pathologists who need to study tissue samples to determine the presence or absence of specific antigens.
02
Medical researchers who need to investigate the expression of certain proteins in different tissues.
03
Clinicians who require additional information about specific biomarkers to aid in diagnosis or treatment decisions.
04
Biomedical scientists who study the localization and function of proteins within cells or tissues.
05
Laboratory technicians who perform immunohistochemistry staining as part of their routine laboratory procedures.
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IHC AMP special stain is a type of staining technique used in immunohistochemistry to detect specific antigens in tissue samples.
Pathologists and laboratory technicians are usually required to perform and file ihc amp special stain.
To fill out ihc amp special stain, the pathologist or technician must carefully follow the staining protocol and record the results accurately.
The purpose of ihc amp special stain is to identify specific proteins or antigens in tissue samples for diagnostic or research purposes.
The information reported on ihc amp special stain includes the type of stain used, the antigens detected, and the results of the staining process.
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