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Lab Dept:Hematologist Name:OSMOTIC FRAGILITYGeneral Information Lab Order Codes:OSFMSynonyms:RBC Fragility; Erythrocyte Fragility; Incubated Osmotic Fragility Codes:85557 Osmotic fragility, RBC; incubatedTest
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How to fill out osmotic fragility

How to fill out osmotic fragility
01
Gather the necessary materials including blood samples, microscope slides, saline solution, osmotic fragility reagent, and a hemocytometer.
02
Prepare the osmotic fragility reagent according to the manufacturer's instructions.
03
Collect a small amount of blood sample using a sterile needle and transfer it into a clean test tube.
04
Centrifuge the blood sample to separate the red blood cells from the plasma.
05
Resuspend the red blood cells in saline solution to create a diluted sample.
06
Prepare a series of tubes with varying concentrations of saline solution.
07
Add a small amount of the diluted red blood cells to each tube.
08
Incubate the tubes at a controlled temperature for a specific time period.
09
After the incubation, carefully observe any changes in the red blood cells, such as hemolysis or increased fragility.
10
Record and analyze the results to determine the osmotic fragility of the blood sample.
Who needs osmotic fragility?
01
Osmotic fragility testing is typically needed in clinical laboratories and diagnostic centers.
02
It is commonly used to diagnose and evaluate various blood disorders including hereditary spherocytosis, thalassemia, and sickle cell disease.
03
Medical professionals, such as hematologists, pathologists, and clinicians, may request osmotic fragility testing to assess a patient's red blood cell membrane integrity.
04
Patients with a family history of blood disorders or individuals experiencing symptoms like anemia, jaundice, or abnormal hemoglobin levels may require osmotic fragility testing.
05
Research institutions and academic settings studying red blood cell physiology and related conditions may also utilize osmotic fragility testing.
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What is osmotic fragility?
Osmotic fragility is a measure of the ability of red blood cells to withstand osmotic stress and is often used in the diagnosis of certain blood disorders.
Who is required to file osmotic fragility?
Medical professionals or laboratories performing blood tests may be required to conduct and report osmotic fragility tests.
How to fill out osmotic fragility?
Osmotic fragility tests involve subjecting red blood cells to varying levels of osmotic stress in a series of solutions, followed by measuring the degree of hemolysis.
What is the purpose of osmotic fragility?
The purpose of osmotic fragility testing is to diagnose conditions such as hereditary spherocytosis and other hemolytic anemias.
What information must be reported on osmotic fragility?
The results of osmotic fragility tests, including the levels of hemolysis at different concentrations of saline solutions, must be reported.
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