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STANDARD OPERATING PROCEDURE FOR ESCHERICHIA COLD (E. COLD) AND TOTAL COLIFORM QUANTIFICATION USING THE IDEA QUANTITY/2000 Systematize of Utah Department of Environmental Quality Division of Water
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01
Start by gathering all the necessary materials, including a sterile petri dish, nutrient agar, a bacterial culture of Escherichia coli E, a sterile inoculating loop, and a Bunsen burner.
02
Ensure a clean and sterile work area by wiping down the bench with a disinfectant solution.
03
Using the Bunsen burner, flame the inoculating loop until it becomes red hot to sterilize it.
04
Carefully open the petri dish lid just enough to expose the agar surface without contaminating it.
05
Dip the sterilized loop into the bacterial culture and gently streak the agar surface in a zigzag pattern.
06
Close the petri dish lid quickly to prevent further contamination
07
Repeat the streaking process if necessary by flaming the loop again and using a different section of the agar surface.
08
Allow the agar to solidify and then incubate the petri dish upside down at the appropriate temperature for Escherichia coli E growth.
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Monitor the growth of Escherichia coli E colonies over a specific period of time and observe any unique characteristics.
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Record your observations and analyze the results accordingly.

Who needs for escherichia coli e?

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Researchers studying Escherichia coli E require it for various experiments and studies.
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Laboratories conducting clinical microbiology tests may need Escherichia coli E for identification and testing purposes.
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Pharmaceutical companies developing antibiotics or other drugs targeting Escherichia coli E may need the bacteria for research and development.
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Food safety laboratories may need Escherichia coli E to test food samples for contamination.
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Academic institutions teaching microbiology or related courses may use Escherichia coli E as a model organism for practical demonstrations and experiments.
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Escherichia coli E (E. coli E) is a specific strain of Escherichia coli bacteria.
Certain healthcare facilities and laboratories may be required to file for Escherichia coli E based on specific guidelines and regulations.
Escherichia coli E forms can typically be filled out online or submitted manually following the provided instructions.
The purpose of filing for Escherichia coli E is to track and monitor the presence of this bacteria for public health and safety reasons.
The required information for Escherichia coli E filing may include details such as sample collection date, testing results, patient information, and related laboratory data.
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