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QUICK REFERENCE Biddle Terminator v1.1 Cycle Sequencing Kit Catalog Numbers 4337449, 4337450, 4337451, 4337452 Pub. No. MAN0015667 Rev. A.0 Note: For safety and biohazard guidelines, see the Safety
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01
Step 1: Start by preparing the reaction mixture. This typically involves combining the necessary components (template DNA, primers, dNTPs, BigDye Terminator mix) in a clean microcentrifuge tube.
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Step 2: Thoroughly mix the reaction mixture by gently pipetting up and down, ensuring all components are well-mixed.
03
Step 3: Prepare the thermal cycler by setting the appropriate cycling parameters. This may involve denaturation, annealing, and extension steps with specific temperatures and durations.
04
Step 4: Once the thermal cycler is ready, load the reaction mixture into the appropriate wells or tubes of the PCR plate.
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Step 5: Place the PCR plate into the thermal cycler and start the program.
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Step 6: After the cycling is complete, remove the plate from the thermal cycler and add the termination mixture (e.g., ethanol) to stop the reaction.
07
Step 7: If necessary, perform purification steps to remove excess terminators and other impurities from the reaction mixture.
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Step 8: Finally, analyze the product using a genetic analyzer to obtain the DNA sequence.
Who needs bigdye terminator v1 1?
01
Scientists and researchers who require accurate DNA sequencing.
02
Laboratories performing genetic analysis and sequencing experiments.
03
Biotechnology companies involved in DNA sequencing services.
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Pharmaceutical companies developing new drugs and therapies.
05
Academic institutions conducting genetic research.
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What is bigdye terminator v1 1?
BigDye Terminator v1.1 is a sequencing mix used for Sanger sequencing applications.
Who is required to file bigdye terminator v1 1?
Researchers and laboratories performing Sanger sequencing that use BigDye Terminator v1.1 are required to file it.
How to fill out bigdye terminator v1 1?
BigDye Terminator v1.1 should be filled out following the manufacturer's protocol and guidelines for sequencing reactions.
What is the purpose of bigdye terminator v1 1?
The purpose of BigDye Terminator v1.1 is to facilitate the sequencing of DNA samples accurately and efficiently.
What information must be reported on bigdye terminator v1 1?
Information such as the DNA sample identifier, sequencing reaction components, and sequencing results must be reported on BigDye Terminator v1.1.
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