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Biochemistry 2000, 39, 10291033 8OxodGTP Incorporation by DNA Polymerase Residue Asn279 1029 Is Modified by Active Site Holly Miller, ×, Rajendra Prasad, Samuel H. Wilson, Francis Johnson, and Arthur
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01
Step 1: Prepare the necessary materials and reagents, including 8-oxodGTP, DNA template, DNA polymerase, nucleotides, buffer solution, and any additional additives.
02
Step 2: Set up a reaction mixture by combining the DNA template, DNA polymerase, nucleotides, buffer solution, and 8-oxodGTP in appropriate ratios. Follow the manufacturer's instructions for the specific enzyme and reaction conditions.
03
Step 3: Incubate the reaction mixture at the recommended temperature for the specified amount of time. This allows for the incorporation of 8-oxodGTP into the growing DNA strand.
04
Step 4: Stop the reaction by adding a stop solution or by heating the mixture to denature the enzymes.
05
Step 5: Analyze the incorporated 8-oxodGTP by techniques such as gel electrophoresis or high-performance liquid chromatography (HPLC).
06
Step 6: Interpret the results and analyze the extent of 8-oxodGTP incorporation, which may provide information about oxidative damage in DNA or its repair mechanisms.

Who needs 8-oxodgtp incorporation by dna?

01
Researchers studying oxidative damage in DNA and its repair mechanisms.
02
Scientists investigating the effects of reactive oxygen species on DNA replication and mutation rates.
03
Experts interested in exploring the role of 8-oxodGTP in various biological processes, including aging, cancer development, and neurodegenerative disorders.
04
Diagnostic laboratories or forensic scientists analyzing DNA samples for oxidative damage.
05
Pharmaceutical companies involved in drug discovery and development targeting DNA repair processes.
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8-oxodGTP incorporation by DNA is a process where the modified nucleotide 8-oxo-2'-deoxyguanosine triphosphate (8-oxodGTP) is inserted into DNA during DNA replication.
Researchers or laboratories conducting studies or experiments involving DNA replication may be required to file 8-oxodGTP incorporation data.
To fill out 8-oxodGTP incorporation data, researchers need to record the amount and locations of 8-oxodGTP incorporated into DNA strands during replication.
The purpose of 8-oxodGTP incorporation by DNA is to study the effects of oxidative stress on DNA replication and potential mutagenic consequences.
Researchers must report the experimental conditions, DNA sequences used, amount of 8-oxodGTP incorporated, and any observed effects on DNA replication.
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Relevant keywords

2000 1991 New_York North_Carolina oxodgtp oxodg polymerase kcat dna datp dntp dctp n279a asn279 alanine r283a arg283 dgtp biol oligonucleotides polymerases nucleotide grollman sawaya misincorporation nm substrates oligonucleotide werneburg shibutani nishi

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