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SAFETY MANUAL AND STANDARD OPERATING PROCEDURES FOR THE OCS Flow Cytometry Core NEW YORK UNIVERSITY SCHOOL OF MEDICINE Old Bellevue C&D building, Room 645 Revised: July 2016 Version: 001 Approval
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01
Begin by properly calibrating your BD FACSAria IIu flow cytometer to ensure accurate results.
02
Prepare your sample by collecting cells of interest and suspending them in an appropriate buffer.
03
Adjust the instrument settings for cell sorting or analysis, including defining gates and selecting fluorochromes for detection.
04
Load the sample onto the sample injection port, taking care not to introduce air bubbles that can affect results.
05
Start the acquisition software and set up the desired data acquisition parameters, such as laser wavelengths, filter settings, and acquisition speed.
06
Run a test sample to optimize the instrument conditions and confirm that cell populations are properly separated.
07
Once the instrument is properly set up, proceed with analyzing or sorting your sample by following the established protocols.
08
After finishing the analysis or sorting, properly clean and decontaminate the instrument to maintain its performance and prevent cross-contamination.
09
Finally, analyze the acquired data using appropriate flow cytometry software to interpret and visualize the results.

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BD FACSAria IIu is used by researchers in various fields, including immunology, cell biology, oncology, and microbiology.
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Scientists and researchers who study cellular processes, immune responses, disease mechanisms, cell proliferation, and differentiation often utilize this cytometer.
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Additionally, facilities involved in clinical diagnostics, drug discovery, and therapeutics development may also require the capabilities of BD FACSAria IIu for their work.
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Individuals or organizations that need high-speed cell sorting, improved sensitivity, and versatility in analyzing multiple parameters can benefit from this instrument.
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BD FACSARIA IIU SORP stands for Becton Dickinson FACSARIA IIU System Operating and Resource Planning.
Only authorized personnel within a company or organization utilizing the BD FACSARIA IIU System are required to file the BD FACSARIA IIU SORP.
The BD FACSARIA IIU SORP form must be completed with accurate information related to the operation and resources planning of the BD FACSARIA IIU System.
The purpose of BD FACSARIA IIU SORP is to document the system operating procedures, resource planning, and utilization of the BD FACSARIA IIU System.
The BD FACSARIA IIU SORP must include details on system operation, resource allocation, maintenance schedules, and any relevant documentation.
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