Get the free Human Urinary NGAL ELISA Test Kit (BIO90NGAL) - Instructions for ...

Arbutus Medical Human NEAL Instructions for Use REF BIO90NGAL 2 96 Well Plate Humanely Enzyme Immunoassay Instructions for Use FOR RESEARCH USE ONLY Not for use in Diagnostic Procedures Arbutus Medical

How to fill out human urinary ngal elisa

How to fill out human urinary NGAL Elisa:

1. Start by ensuring that you have all the necessary materials and reagents for the NGAL Elisa assay. This may include the NGAL Elisa kit, microplate reader, pipettes, and appropriate buffers.
2. Carefully review the instructions provided with the NGAL Elisa kit. Familiarize yourself with the components, their storage conditions, and any specific handling instructions.
3. Ensure that the samples to be tested are properly collected, stored, and labeled. Follow the recommended guidelines for sample collection, including any necessary processing steps such as centrifugation or freezing.
4. Thaw frozen samples and mix them well before starting the assay. Allow the samples to come to room temperature, if needed.
5. Prepare the NGAL Elisa plate by adding the appropriate controls and standards provided in the kit. Follow the instructions for diluting the standards and samples to the recommended concentrations. Use a multichannel pipette to ensure accuracy and efficiency.
6. Carefully pipette the diluted standards and samples into their designated wells on the NGAL Elisa plate. Be sure to follow the recommended order and avoid cross-contamination between wells. It is helpful to use a plate template or guide to keep track of the samples and their positions on the plate.
7. Seal the Elisa plate with the provided plate sealer to prevent evaporation and leakage. Gently tap the plate to ensure proper mixing of the samples and reagents inside each well.
8. Incubate the plate according to the recommended time and temperature mentioned in the kit's instructions. This will allow the NGAL antigen and any NGAL antibodies in the sample to interact and form immune complexes.
9. After the incubation period, carefully remove the plate sealer and discard any remaining liquid from the wells. Wash the plate thoroughly with the provided wash buffer to remove any unbound substances.
10. Add the NGAL detection reagent to each well, ensuring that it is evenly distributed. Incubate the plate again under the recommended conditions.
11. Repeat the washing step to remove any unbound detection reagent.
12. Add the substrate solution to each well, following the kit's instructions. Allow the color to develop for the recommended incubation time.
13. Stop the reaction by adding the stop solution to each well. The color will change from blue to yellow.
14. Use a microplate reader to measure the absorbance of each well at the appropriate wavelength. Follow the manufacturer's instructions for data collection and analysis.

Who needs human urinary NGAL Elisa:

1. Researchers and scientists studying kidney diseases and injuries often utilize human urinary NGAL Elisa to measure NGAL levels in urine samples. This biomarker helps in the early detection of acute kidney injury and monitoring its progression.
2. Clinicians and healthcare professionals involved in nephrology may use human urinary NGAL Elisa to aid in the diagnosis, prognosis, and monitoring of various kidney disorders. NGAL levels can provide valuable insights into the severity and progression of kidney diseases.
3. Pharmaceutical companies and biotech firms developing drugs or therapies targeting renal diseases may require human urinary NGAL Elisa for preclinical and clinical trials. NGAL levels can serve as a useful biomarker to assess the effectiveness and potential side effects of the interventions.

In summary, filling out a human urinary NGAL Elisa involves careful adherence to the kit instructions, proper sample handling, and accurate pipetting techniques. The NGAL Elisa assay is valuable for researchers, healthcare professionals, and pharmaceutical companies involved in studying, diagnosing, and treating kidney diseases.

For pdfFiller’s FAQs

What is human urinary ngal elisa?

Human urinary NGAL ELISA is a test used to measure levels of neutrophil gelatinase-associated lipocalin (NGAL) in urine, which can indicate kidney damage or dysfunction.

Who is required to file human urinary ngal elisa?

Healthcare professionals or researchers who need to assess kidney function or diagnose kidney injury may be required to perform or interpret human urinary NGAL ELISA tests.

How to fill out human urinary ngal elisa?

To fill out a human urinary NGAL ELISA test, follow the instructions provided with the test kit and ensure proper handling of samples and accurate recording of results.

What is the purpose of human urinary ngal elisa?

The purpose of human urinary NGAL ELISA is to measure levels of NGAL in urine as a potential indicator of kidney injury, acute kidney disease, or chronic kidney conditions.

What information must be reported on human urinary ngal elisa?

The test results, patient information, date and time of sample collection, any medications or medical conditions affecting kidney function should be reported on human urinary NGAL ELISA forms.

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