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Genomics Proteomics Bioinformatics 11 (2013) 182194 Genomics Proteomics Bioinformatics www.elsevier.com/locate/gpb www.sciencedirect.com ORIGINAL RESEARCH Candidate Biomarker Discovery for Angiogenesis
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01
Start by preparing the sample for tandem MS2 sequencing.
02
Next, extract the proteins of interest from the sample using techniques like gel electrophoresis or liquid chromatography.
03
Digest the proteins into peptides using enzymes like trypsin.
04
Prepare the tandem MS2 instrument for analysis by setting the appropriate parameters and calibrating the instrument.
05
Load the peptides onto the tandem MS2 instrument and run the analysis.
06
Collect the MS2 spectra data generated by the instrument.
07
Process and analyze the MS2 spectra data using appropriate software to identify and sequence the peptides.
08
Annotate and interpret the tandem MS2 sequencing results to determine the amino acid sequence of the peptides.
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Validate the tandem MS2 sequencing results through additional experiments or by comparing with known sequences.
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Document and report the tandem MS2 sequencing results for further analysis or publication.

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01
Biomedical researchers studying protein structure and function.
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Forensic scientists analyzing trace amounts of proteins in crime scenes.
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Researchers studying protein-protein interactions.
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Biochemists studying post-translational modifications of proteins.
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Biotechnology companies developing protein-based products.
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Clinical laboratories conducting diagnostic tests.
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Tandem MS2 sequencing is in the field of proteomics.
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Tandem MS2 sequencing can be filled out by entering the relevant data and information in the designated fields.
The purpose of tandem MS2 sequencing is to analyze and identify proteins in biological samples.
Information such as amino acid sequences, protein modifications, and spectral data must be reported on tandem MS2 sequencing.
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