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Commercial AcTEV Pro In-House TEV Pro Figure 3. 16. Commercial AcTEV protease versus In-house produced 6xHis TEV protease Activity Assay. 7. 3. 1. 4. 3 Second Round IMAC TEV protease to purify the EF2-GGC peptide. A B Figure 3. 8. Second round of IMAC for purification of the EF2-GGC peptide. 84 EF2-GGC Peptide Amino Acid Sequence. 84 6xHis CA TEV cleavage site Amino Acid Sequence. 85 General Ni-NTA Resin Washing. Optimization of Ni-NTA IMAC. Section 3. 1. 2. 1 3. 5. TEV protease cleavage of...
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