Get the free RNAi in Plants: An Argonaute-Centered View - Plant Cell
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In plants several sRNA species that differ in biogenesis and functions have been characterized. The predominant sRNA species include microRNAs miRNAs generated by DCL1 Reinhart et al. 2002 Kurihara and Watanabe 2004 Qi et al. 2005 trans-acting small interfering RNAs ta-siRNAs processed by DCL4 Peragine et al. 2004 Vazquez et al. 2004 Gasciolli et al. 2005 Xie et al. 2005 and heterochromatic siRNAs hc-siRNAs produced by DCL3 Xie et al. 2004 Qi et al. 2005 Henderson et al. 2006. Additional...
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How to fill out rnai in plants an
How to fill out rnai in plants an
01
To fill out RNAi in plants, follow these steps:
02
Start by selecting the target gene for RNAi silencing.
03
Design and synthesize a small RNA molecule called a short-hairpin RNA (shRNA) that is complementary to the target gene sequence.
04
Insert the shRNA sequence into a suitable RNAi vector, such as a binary vector.
05
Introduce the RNAi vector into Agrobacterium tumefaciens, a bacterium commonly used for plant transformation.
06
Prepare the plant tissue, such as leaves or callus, for transformation.
07
Use a genetic engineering technique, such as Agrobacterium-mediated transformation or biolistic bombardment, to deliver the RNAi vector into the plant cells.
08
Allow the transformed plant cells to regenerate into whole plants under appropriate culture conditions.
09
Screen and select the successfully transformed plants that contain the RNAi construct.
10
Verify the presence of the shRNA and its expression in the selected plants using molecular techniques, such as PCR or Northern blotting.
11
Perform functional analysis or phenotype observation to confirm the RNAi-mediated silencing of the target gene in the transformed plants.
Who needs rnai in plants an?
01
RNAi in plants can be beneficial for various individuals and entities, including:
02
- Plant researchers studying gene function and regulation.
03
- Breeders interested in developing improved crop varieties with specific traits.
04
- Agricultural companies aiming to enhance crop yield, quality, and resistance to pests or diseases.
05
- Farmers seeking to improve the characteristics of their crops for increased productivity and profitability.
06
- Pharmaceutical companies looking to produce plant-based products with specific medicinal properties.
07
- Environmental scientists investigating the impact of gene silencing on plant ecosystems and biodiversity.
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What is rnai in plants an?
RNAi (RNA interference) in plants is a natural process that involves the silencing of specific genes by the introduction of double-stranded RNA molecules.
Who is required to file rnai in plants an?
Researchers and institutions conducting experiments involving RNA interference in plants are required to file RNAi notifications.
How to fill out rnai in plants an?
To fill out an RNAi notification in plants, researchers must provide details on the genes being targeted, the method of gene silencing, and any potential off-target effects.
What is the purpose of rnai in plants an?
The purpose of RNAi in plants is to study the function of specific genes, develop new plant traits, and potentially improve crop yields and sustainability.
What information must be reported on rnai in plants an?
Researchers must report the specific gene(s) being targeted, the method of gene silencing, any off-target effects, and the potential impact on plant growth and development.
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