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Occurs when formation of ES breakdown of ES. k1 E S k-1 k2 ES ES E S / k-1 k2 / k1 Define constant Km Michaelis constant Km k-1 k2 / k1 Therefore ES E S / Km Solve for ES. 6. 1. 18 4. Lyases Catalyze lysis of a substrate generating a double bond in a nonhydrolytic nonoxidative elimination i.e. nonhydrolytic nonoxidative lysis that yields a double bond EC 4. BCMB 3100 Chapters 6 7 8 Enzyme Basics Six Classes IUBMB Kinetics Michaelis-Menten Equation Vo Km Vmax Kcat Lineweaver-Burk Plot...
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