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26 ix CU DD SEM FTIR XRD Mw MHA MIC C. albicans E.coli B. cereus S. aureus P. aeruginosa E.facealis mm PM P. 23 Figure 3. 5 XRD diffractogram of 0. 20g CU blended bioceramic. 25 Figure 3. 6 SEM micrograph 0. 20g CU blended bioceramic at x100. 22 c Enterococcus faecalis. d Bacillus cereus. 22 e Pseudomonas aeruginosa. b Candida albicans. 23 Figure 3. There are also blends of chitosan/alginate sponges Shapiro 1997 Kofuji 2001 Kataoka 2001 Singla 2001 Kumar 2000 Coppi 2002 Lai 2003. No et al....
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01
Prepare the chitosan-sea sample by mixing chitosan and sea extract in a desired ratio.
02
Choose the appropriate method to determine the anti-microbial activity, such as agar diffusion method or microdilution method.
03
Sterilize the required equipment and media before starting the experiment.
04
Prepare the agar plates or microplates with appropriate growth medium and inoculate them with the test microorganisms.
05
Apply the chitosan-sea sample to the wells or discs on the agar plates, ensuring proper distribution.
06
Incubate the plates at the optimal temperature and time for microbial growth.
07
Measure the zone of inhibition or determine the minimum inhibitory concentration by observing the growth or lack thereof around the chitosan-sea sample.
08
Record the results and analyze the data to determine the anti-microbial activity of chitosan-sea.
09
Repeat the experiment for multiple concentrations or variations to validate the findings.
10
Compile the data and draw conclusions about the anti-microbial activity of chitosan-sea.

Who needs anti-microbial activity of chitosan-sea?

01
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02
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04
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05
Environmental scientists exploring eco-friendly anti-microbial solutions
06
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07
Agricultural experts investigating natural methods for crop protection
08
Veterinary professionals addressing antimicrobial resistance in animals
09
Pharmacologists researching potential applications of chitosan-sea in medicine
10
Entrepreneurs and innovators interested in developing chitosan-sea-based products
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Chitosan-sea exhibits anti-microbial properties that help inhibit the growth of bacteria and fungi.
Manufacturers, distributors, or researchers working with chitosan-sea products may be required to file the anti-microbial activity data.
To fill out the anti-microbial activity of chitosan-sea, a standardized form may need to be completed with relevant data such as testing methodology, results, and any observed effects.
The purpose of documenting the anti-microbial activity of chitosan-sea is to ensure product safety and effectiveness, as well as to provide transparency to consumers and regulatory bodies.
Information that must be reported can include the specific microorganisms tested, inhibition zones, minimum inhibitory concentrations, and any relevant research findings.
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