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As DH5 does not have laclq IPTG is not required for blue-white screening. 8. Incubate the plate at 37 C overnight. 5 g/L 0. 186 g/L 2. 4 g/L 4 g/L tryptone yeast extract NaCl KCl MgSO4 anhydrous glucose Ver. 1. 0 Materials to be supplied by user LB plates with antibiotic 42 C water bath Sterile spreaders 15 ml sterilized-polypropylene culture tubes Ice bucket with ice 37 C shaker 37 C incubator If blue-white screening is required to select transformants 20 mg/ml X-Gal in dimethylformamide DMF...
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