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To directly address the supposition that active MMP-2 kills ECs the influence of the same examined following incubation of 2h 8h and 24h Figure 5D. The higher concentration of activated form led to cell death at a later time 8-24h confirming the ability of fully active MMP-2 to cause ECs apoptotic death. Of note in the presence of the high concentration of commercial non-activated MMP-2 which contained pro-enzyme as well as a large fraction of fully active MMP-2 the level of apoptosis after...
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