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HCMV tegument protein pp71 is directly cleaved by GrM. A HCMV-purified lysates 5 mg were incubated with GrM 100 nM or GrM-SA 100 nM for 2 h at 37 C. To investigate whether GrM-cleaved pp71 is functionally active truncated pp71 variants pp711 439 and pp71440 559 that mimic GrM-cleaved pp71 fragments were generated Fig. Tegument pp71 during HCMV infection under physiological conditions. GrM directly cleaves pp71 after Leu439 FIGURE 3. Upon infection tegument pp71 Acknowledgments We thank Dr. T....
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Gather the necessary equipment such as a sterile culture plate, media, and pipettes.
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Inoculate the culture plate with the cytomegalovirus-infected cells by carefully pipetting a small amount onto the surface of the media.
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Incubate the culture plate at the optimal temperature and conditions for the cells to grow.
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Monitor the growth and health of the cells by regularly checking for signs of resistance to the cytomegalovirus infection.
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Analyze and record the results of the resistance levels in the cells over time.
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Repeat the process to validate the findings and ensure consistency in the resistance of the cells.
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What is cytomegalovirus-infected cells resist t?
Cytomegalovirus-infected cells resist t refers to cells that are resistant to the effects of cytomegalovirus infection.
Who is required to file cytomegalovirus-infected cells resist t?
Researchers or healthcare providers studying cytomegalovirus infection may be required to file information on cytomegalovirus-infected cells resist t.
How to fill out cytomegalovirus-infected cells resist t?
The form for cytomegalovirus-infected cells resist t may require information on the resistance mechanisms observed in infected cells.
What is the purpose of cytomegalovirus-infected cells resist t?
The purpose of documenting cytomegalovirus-infected cells resist t is to understand how cells can resist the effects of cytomegalovirus infection.
What information must be reported on cytomegalovirus-infected cells resist t?
Information such as the type of cells studied, methods used to assess resistance, and any observed resistance mechanisms may need to be reported on cytomegalovirus-infected cells resist t.
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