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Protocol Sheet Applied Biosystems 7900HT real-time PCR run setup instructions for Microbial DNA qPCR Arrays Important points before starting Please read the handbook supplied with the Microbial DNA qPCR Array paying careful attention to the Safety Information and Important Notes sections before beginning this procedure. Qiagen.com or can be requested from QIAGEN Technical Services or your local distributor. Trademarks QIAGEN QIAGEN Group Applied Biosystems Applera Corporation or its...
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Step 1: Turn on the Applied Biosystems 7900HT Real-Time system.
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Step 2: Prepare the reaction mix by following the protocol provided by the manufacturer.
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Step 3: Load the reaction mix into the appropriate wells in the reaction plate.
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Step 4: Place the reaction plate into the Applied Biosystems 7900HT Real-Time system.
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Step 5: Set up the desired parameters for the real-time PCR reaction, such as temperature and cycling conditions.
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Step 6: Start the real-time PCR run by initiating the software and selecting the appropriate program.
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Step 7: Monitor the real-time amplification curve and collect data as needed.
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Step 8: Analyze the data using the software provided by Applied Biosystems or other compatible analysis software.
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Step 9: Interpret the results and draw conclusions based on the real-time PCR data obtained in the Applied Biosystems 7900HT Real-Time system.
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Step 10: Clean and maintain the system according to the manufacturer's instructions for optimal performance.
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