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IV. Materials Provided Catalog Component Name 1 Slide Box 2 Slide Box 1 QAM-CYT-3S Mouse Cytokine Array 3 Glass Slide 2 QA-SDB Quantibody Sample Diluent 3 AA-WB1-30ML 20X Wash Buffer I 30 ml 5 QAM-CYT-3-STD Standard Mix 1 Vial Antibody Cocktail 7 QA-CY3E Cy3 equivalent dye-conjugated Streptavidin 8 QA-SWD Slide Washer/Dryer 1 x 30 ml Tube 9 QA-ADH Adhesive Film 15 ml 2 x 30 ml 1-25 l 5 l 2 x 5 l 4 slide kits are comprised of 2 separate 2 slide kits. December 20 2012 Blood 120 26. http //dx....
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How to fill out quantibody mouse cytokine array
How to fill out quantibody mouse cytokine array
01
Step 1: Prepare the samples and dilutions. Ensure that the samples are properly stored and thawed.
02
Step 2: Prepare the Antibody Cocktail Mix. Follow the instructions provided in the kit to create the required mix.
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Step 3: Add the Antibody Cocktail Mix to the array slide wells. Make sure to add the appropriate volume as specified in the kit instructions.
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Step 4: Incubate the array slide overnight at the recommended temperature.
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Step 5: Wash the array slide carefully according to the provided protocol to remove any unbound materials.
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Step 6: Apply the Detection Antibody Mix to the array slide wells and incubate for the specified time.
07
Step 7: Wash the array slide again to remove any unbound detection antibodies.
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Step 8: Apply the Streptavidin-HRP solution to the array slide wells and incubate for the recommended duration.
09
Step 9: Wash the array slide thoroughly to remove any excess Streptavidin-HRP.
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Step 10: Prepare the Substrate Solution and apply it to the array slide wells.
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Step 11: Allow the color to develop for the specified time and stop the reaction by adding the Stop Solution.
12
Step 12: Use a microarray scanner or imaging system to capture the images of the array slide.
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Step 13: Analyze the obtained data using the recommended software or analysis tool.
Who needs quantibody mouse cytokine array?
01
Researchers studying mouse cytokines and their expression patterns.
02
Scientists investigating immunology and inflammatory responses in mice.
03
Drug development companies conducting preclinical studies on mouse models.
04
Biotech companies involved in mouse cytokine biomarker research.
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What is quantibody mouse cytokine array?
Quantibody mouse cytokine array is a multiplex protein array designed to detect and quantify multiple cytokines in a single sample.
Who is required to file quantibody mouse cytokine array?
Researchers and scientists working with mouse models or studying cytokine expression are required to use and file quantibody mouse cytokine array.
How to fill out quantibody mouse cytokine array?
Quantibody mouse cytokine array can be filled out by adding the sample to the designated areas on the array slide and following the manufacturer's protocol for detection and analysis.
What is the purpose of quantibody mouse cytokine array?
The purpose of quantibody mouse cytokine array is to analyze and quantify the expression levels of multiple cytokines in a single sample, providing valuable information for research and study.
What information must be reported on quantibody mouse cytokine array?
The information reported on quantibody mouse cytokine array includes the expression levels of various cytokines present in the sample, along with any other relevant data such as sample details and experimental conditions.
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