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Figure 2. CRISPR/Cas9 System Applications A. Wild-type Cas9 nuclease site specifically cleaves double-stranded DNA activating double-strand break repair machinery. To achieve site-specific DNA recognition and cleavage Cas9 must be complexed with both a crRNA and a separate trans-activating crRNA tracrRNA or trRNA that is partially complementary to the crRNA. In fact even fully Cas9 and CRISPR as a New Tool in Molecular Biology The simplicity of the type II CRISPR nuclease with only three...
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