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Laboratory Procedure Manual
Analyte: Tissue Transglutaminase Assay (IGA)
Matrix: Serum
Method:
IGA (ELISA)
Method No:
Revised:as performed by:contact:Important Information for Users Mayo Clinic
Rochester,
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How to fill out analyte tissue transglutaminase assay
How to fill out analyte tissue transglutaminase assay
01
Obtain the necessary materials for the assay, including the analyte tissue transglutaminase assay kit.
02
Familiarize yourself with the kit's instructions and safety precautions.
03
Prepare the samples by collecting tissue samples and homogenizing them in a suitable buffer.
04
Follow the kit's protocol to extract the analyte of interest from the tissue samples.
05
Prepare the assay plate by adding the appropriate controls, standards, and samples.
06
Add the assay reagents according to the kit's instructions and incubate for the specified time.
07
Use the provided detection system to measure the analyte levels in the samples.
08
Record and interpret the assay results based on the kit's guidelines.
09
Dispose of any hazardous waste generated during the assay following proper safety procedures.
10
Clean and sanitize the work area and equipment used in the assay.
Who needs analyte tissue transglutaminase assay?
01
Individuals suspected of having autoimmune diseases such as celiac disease.
02
Patients with gastrointestinal symptoms and malabsorption issues.
03
Individuals with a family history of celiac disease or gluten sensitivity.
04
Those who have previously tested positive for other celiac disease markers.
05
Individuals on a gluten-free diet who want to monitor their gluten exposure levels.
06
Researchers studying autoimmune diseases and related biomarkers.
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