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GE HealthcarepGEX3X
GST Expression Vector
Product Specification Sheet
Code:28954654
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For research use only.
Not recommended or intended for diagnosis of disease in
humans or animals.
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How to fill out gst expression vector
How to fill out gst expression vector
01
Start by obtaining the GST expression vector.
02
Prepare the DNA fragment or gene of interest that you wish to express using the GST system.
03
Double-check the compatibility of your DNA fragment with the chosen GST expression vector.
04
Perform a restriction enzyme digestion of both the vector and the DNA fragment to generate compatible ends for ligation.
05
Purify the digested vector and DNA fragment to remove any contaminants or enzymes.
06
Mix the purified vector and DNA fragment in the proper ratio and incubate them with DNA ligase to catalyze the ligation reaction.
07
Transform the ligated DNA into competent bacterial cells using a suitable transformation method (e.g., heat shock or electroporation).
08
Plate the transformed cells onto selective agar medium containing the appropriate antibiotic to select for cells that have taken up the GST expression vector.
09
Incubate the plates at the appropriate temperature overnight to allow bacterial colonies to grow.
10
Pick colonies from the plates and inoculate them into liquid culture media to propagate the bacteria and the GST expression vector.
11
Perform plasmid DNA extraction from the bacterial culture to isolate the GST expression vector.
12
Verify the presence and integrity of the desired DNA fragment in the extracted GST expression vector using techniques like restriction enzyme digestion, PCR, or DNA sequencing.
13
Use the purified GST expression vector for downstream applications, such as protein expression, purification, and analysis.
Who needs gst expression vector?
01
Researchers studying protein function and expression.
02
Scientists interested in producing recombinant proteins for various applications.
03
Biotechnologists involved in protein purification and analysis.
04
Laboratories conducting molecular biology and genetic engineering experiments.
05
Pharmaceutical companies and drug discovery researchers.
06
Academic institutions and research centers focusing on molecular biology and biotechnology.
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