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G & M Procter Ltd. Certificate of Analysis PRODUCTPO1191S MUELLERHINTONAGAR 140 MM (4MM SCUD.) 1×5 PLT(14CM)LOT NUMBER 1249695 EXPIRY DATE 2013.05.02 PRODUCTION DATE 2013.02.28 TEST DATE 2013.02.28
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01
Gather all the required materials: Mueller-Hinton Agar 140 mm 4mm plate, inoculating loop or swab, bacterial culture to be tested.
02
Start by labeling the bottom side of the agar plate with the necessary information such as the date, sample name, or any other relevant details.
03
Open the agar plate carefully without touching the agar surface and ensure it remains sterile.
04
Take the inoculating loop or swab and dip it into the bacterial culture to obtain a sample for testing.
05
Carefully streak the inoculating loop or swab in a zigzag motion across one section of the agar plate, also known as the primary streak.
06
Flame sterilize the loop or swab before proceeding to the next section of the plate and make a secondary streak by streaking perpendicular to the primary streak.
07
Repeat the streaking process for subsequent sections of the plate, ensuring each section is labeled and streaked in a different direction.
08
Once all the sections have been streaked, securely close the agar plate. It is important to ensure it remains sealed to maintain sterility.
09
Incubate the agar plate upside down in an incubator set to the appropriate temperature for the bacterial culture being tested.
10
After the required incubation period, carefully observe the agar plate for bacterial growth and record any visible colonies or zones of inhibition, if applicable.

Who needs mueller-hinton-agar 140 mm 4mm?

01
Mueller-Hinton Agar 140 mm 4mm is commonly used in microbiology laboratories for the purpose of performing antibiotic susceptibility testing.
02
It is specifically designed to meet the requirements of the standardized Kirby-Bauer disc diffusion method for determining the susceptibility of bacteria to various antibiotics.
03
Medical professionals, researchers, and scientists in the field of microbiology typically utilize Mueller-Hinton Agar 140 mm 4mm for accurate antimicrobial susceptibility testing.
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It is a type of agar used in microbiology for antibiotic susceptibility testing.
Laboratories conducting antibiotic susceptibility testing are required to use Mueller Hinton agar.
The agar is prepared according to specific guidelines and is used to culture bacteria for susceptibility testing.
The purpose of using Mueller Hinton agar is to provide a standardized medium for antibiotic susceptibility testing of bacteria.
The results of the antibiotic susceptibility testing, including the type of bacteria tested and the effectiveness of various antibiotics.
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