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Certificate of AnalysispGL4.26 luc2/mind/Hydro Vector: Part No. E844APart# 9PIE844 Revised 10/16Size 20gInstructions for use of this product can be found in the pGL4 Luciferase Reporter Vectors Technical
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How to fill out 26luc2minphygro vector
01
Obtain the 26luc2minphygro vector from a reliable source.
02
Thaw the vector on ice to ensure its stability.
03
Prepare a suitable bacterial strain for transformation.
04
Add 2 μl of vector to 50 μl of competent cells.
05
Incubate the mixture on ice for 30 minutes.
06
Heat shock the cells at 42°C for 45 seconds.
07
Immediately transfer the cells back to ice for 2 minutes.
08
Add 950 μl of room temperature SOC medium to the cells.
09
Shake the tubes at 37°C for 45 minutes at 220 rpm.
10
Plate the appropriate dilutions onto selective agar plates.
11
Incubate the plates overnight at the suitable temperature.
12
Screen the colonies for desired insert using appropriate methods.
Who needs 26luc2minphygro vector?
01
Researchers working in molecular biology.
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Those interested in luciferase-based assays.
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Laboratories conducting genetic engineering experiments.
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Individuals conducting functional genomics research.
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Scientists exploring promoter activity.
07
Researchers interested in studying cellular signal transduction pathways.
08
Biotechnologists involved in recombinant protein production.
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