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NameClassDateSkills WorksheetConcept Mapping
Using the terms and phrases provided below, complete the concept map showing
the characteristics of organic compounds.carbohydrateslipidsphospholipidsDNAmonosaccharidespolysaccharidesenzymesnucleic
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How to fill out nucleotides
How to fill out nucleotides
01
Start by gathering the necessary materials: a DNA sequence, a primer, DNA polymerase, nucleotides (A, T, C, G), and a buffer solution.
02
Set up a PCR reaction by combining the DNA sequence, primer, DNA polymerase, nucleotides, and buffer solution in a PCR tube.
03
Place the PCR tube in a thermal cycler machine and program the desired temperature cycles for DNA amplification.
04
During the denaturation step, heat the mixture to separate the DNA double strands.
05
During the annealing step, cool the mixture to allow the primer to bind to the DNA template.
06
During the extension step, raise the temperature to enable DNA polymerase to synthesize new DNA strands using the nucleotides.
07
Repeat the temperature cycles as programmed to amplify the DNA sequence.
08
After the PCR reaction is complete, analyze the amplified DNA by methods such as gel electrophoresis or DNA sequencing.
Who needs nucleotides?
01
Scientists and researchers studying genetics or molecular biology often require nucleotides for various experiments and studies.
02
Medical professionals use nucleotides in molecular diagnostic tests to detect genetic diseases or mutations.
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Pharmaceutical companies may need nucleotides for drug discovery, development, and testing.
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Agricultural researchers utilize nucleotides for genetic improvement of crops and livestock breeding.
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Forensic experts rely on nucleotides for DNA profiling and identification purposes.
06
Biotechnology companies and genetic engineering industries utilize nucleotides for genetic manipulation or modifications.
07
Educational institutions and universities use nucleotides in genetics and molecular biology courses and research projects.
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