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DRINKING WATER PROTECTIONDrinking Water Sample Collection and Preservation Total Coliform Reference Method SM 9223BREAD INSTRUCTIONS CAREFULLY. SAMPLES MAY BE REJECTED IF ALL INSTRUCTIONS ARE NOT
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How to fill out total coliform reference method

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Step 1: Gather all necessary materials and equipment including sample containers, sterile gloves, pipettes, petri dishes, and a water sample.
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Step 2: Sterilize all equipment using an autoclave or by using a sterilizing solution.
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Step 3: Collect a representative water sample from the desired location using the appropriate sampling method.
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Step 4: Add the water sample to the pre-sterilized sample container, making sure to follow any specific instructions for the method being used.
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Step 5: Incubate the sample at the appropriate temperature and time, as specified by the reference method being followed.
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Step 6: Prepare the growth media according to the reference method's instructions.
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Step 7: Using the aseptic technique, transfer a small volume of the incubated sample onto the prepared growth media.
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Step 8: Incubate the petri dishes at the specified temperature and time.
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Step 9: After incubation, count the total number of coliform colonies present on the petri dishes.
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Step 10: Calculate the coliform concentration in the original water sample based on the dilution factor and the number of colonies counted.
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Step 11: Record and report the results according to the reference method guidelines.

Who needs total coliform reference method?

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Total coliform reference method is needed by laboratories and organizations involved in water quality testing and monitoring.
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Environmental agencies, water treatment facilities, and research institutions also require this method to ensure the safety and quality of drinking water sources.
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Regulatory bodies and governments may mandate the use of the total coliform reference method to enforce compliance with water quality standards and regulations.
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Furthermore, industries such as food and beverage production, agriculture, and recreational water facilities may utilize this method to assess the microbial contamination level in their processes and products.
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Total coliform reference method is a standardized method used to detect and quantify the presence of coliform bacteria in a water sample.
All water testing facilities and laboratories that are responsible for testing water quality are required to file total coliform reference method.
Total coliform reference method should be filled out according to the specific guidelines provided by the regulatory authorities overseeing water quality standards.
The purpose of total coliform reference method is to ensure the safety of drinking water by monitoring and measuring the levels of coliform bacteria present in the water supply.
The total coliform reference method must include details about the water sample, testing procedures, results, and any corrective actions taken based on the results.
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