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Antibody Mouse Cytokine Array 1 Quantitative measurement of 20 mouse cytokines Catalog #: QAMCYT1 User Manual Last revised May 2015 Caution: Extraordinarily useful information enclosed ISO 13485 Certified
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How to fill out quantibody mouse cytokine array

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How to fill out quantibody mouse cytokine array:

01
Start by preparing your samples. You will need to collect mouse serum or plasma, as well as any other biological fluids or tissue lysates you wish to analyze.
02
Thaw the quantibody array slide at room temperature for about 15 minutes. Make sure to handle the slide carefully to avoid scratching the surface.
03
Once thawed, place the slide on a flat surface and remove the adhesive film covering the array spots. Be cautious not to touch or contaminate the spots.
04
Prepare the blocking buffer provided in the kit according to the instructions. This buffer will help prevent non-specific binding of proteins to the array surface.
05
Add the blocking buffer onto the slide, making sure to cover all the array spots. Incubate the slide for about 30 minutes at room temperature to allow for proper blocking.
06
While the slide is incubating, prepare your samples for loading. Dilute your samples using the dilution buffer provided in the kit, following the instructions for the desired sample volume.
07
After the incubation period, carefully remove the blocking buffer from the slide. Gently tap the slide on a clean tissue paper or paper towel to remove any excess liquid.
08
Load your diluted samples onto the array spots. Make sure to pipette the samples onto the spots accurately and avoid bubble formation.
09
Once the samples are loaded, carefully place the adhesive film back onto the slide, covering all the array spots. Make sure there are no wrinkles or air bubbles in the film.
10
Incubate the slide in a humidity chamber or a slide tray and shake gently for 2 hours at room temperature. This will allow for proper binding of the analytes in your samples to the array spots.
11
After the incubation, remove the adhesive film and carefully wash the slide using the wash buffer provided in the kit. Follow the instructions for the number of washes and incubation times.
12
Finally, scan the quantibody array slide using a compatible microarray scanner. Follow the scanner's instructions for proper settings and image acquisition.

Who needs quantibody mouse cytokine array:

01
Researchers studying mouse immune responses and cytokine signaling pathways can benefit from using the quantibody mouse cytokine array. It allows for the simultaneous detection and quantification of multiple cytokines in a single experiment.
02
Pharmaceutical companies or biotech companies developing new drugs or therapies can use the quantibody array to assess the efficacy of their treatments in modulating cytokine levels in mouse models.
03
Veterinarians or animal researchers working with mice can utilize the quantibody array to evaluate cytokine profiles and immune system function in different disease models or drug treatments.
04
Academic institutions or research organizations conducting mouse cytokine profiling studies can employ the quantibody array to obtain comprehensive cytokine expression data quickly and efficiently.
05
Scientists interested in studying the immune response and cytokine release in various mouse disease models, such as cancer, inflammation, infection, or autoimmune diseases, can utilize the quantibody array for their research purposes.
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Quantibody Mouse Cytokine Array is a multiplex ELISA-based array used for detecting multiple cytokines in a single sample.
Researchers or scientists conducting studies on mouse cytokines are required to file Quantibody Mouse Cytokine Array.
Quantibody Mouse Cytokine Array can be filled out by following the instructions provided by the manufacturer of the kit.
The purpose of Quantibody Mouse Cytokine Array is to measure the levels of multiple cytokines simultaneously in a single sample, providing valuable data for research purposes.
The information reported on Quantibody Mouse Cytokine Array includes the levels of various cytokines present in the sample.
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