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Certificate of AnalysispGL4.15 luc2P/Hydro Vector: Part No. E670APart# 9PIE670 Revised 10/16Size 20gInstructions for use of this product can be found in the pGL4 Vectors Technical Manual #TM259, available

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How to fill out 15luc2phygro vector

01

Step 1: Open the 15luc2phygro vector and extract the DNA sequence from the plasmid.

02

Step 2: Design and synthesize the desired gene or DNA fragment that you want to insert into the vector.

03

Step 3: Prepare the DNA fragment by amplifying it using PCR or any other suitable method.

04

Step 4: Digest the 15luc2phygro vector and the amplified DNA fragment using appropriate restriction enzymes.

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Step 5: Use DNA ligase to ligate the digested DNA fragment into the digested vector.

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Step 6: Transform the ligated vector into competent bacterial cells using a suitable transformation method.

07

Step 7: Plate the transformed cells onto selective agar plates containing the appropriate antibiotic.

08

Step 8: Incubate the plates overnight at the optimal temperature for bacterial growth.

09

Step 9: Pick a colony from the plate and perform colony PCR to confirm the presence of the desired DNA fragment in the vector.

10

Step 10: Verify the complete sequence of the vector by DNA sequencing if necessary.

Who needs 15luc2phygro vector?

01

The 15luc2phygro vector is commonly used in molecular biology research and is needed by scientists and researchers who are studying luciferase gene expression or working with luciferase reporter assays.

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It is also required by scientists who want to introduce a selectable marker (hygromycin resistance) into their experimental system.

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Furthermore, researchers who aim to study gene expression or perform functional studies in mammalian cells often use the 15luc2phygro vector as a convenient tool.

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What is 15luc2phygro vector?

15luc2phygro vector is a plasmid vector used for gene expression in mammalian cells.

Who is required to file 15luc2phygro vector?

Researchers conducting experiments involving gene expression in mammalian cells may be required to file 15luc2phygro vector.

How to fill out 15luc2phygro vector?

To fill out 15luc2phygro vector, one must provide information about the gene of interest, promoter region, and other relevant genetic elements.

What is the purpose of 15luc2phygro vector?

The purpose of 15luc2phygro vector is to facilitate the study of gene expression and regulation in mammalian cells.

What information must be reported on 15luc2phygro vector?

On 15luc2phygro vector, one must report details about the gene being expressed, the promoter controlling its expression, and any other genetic elements included in the vector.

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