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Sequencing with Next era Rapid Capture Enrichment Kits RESEARCH USE ONLY
Next era Rapid Capture Enrichment kits are available in
various configurations:
Samples
8
24
48
72
24
48
96
288EnrichmentReactions
8
8
8
8
2
4
8
24Plexity
1
3
6
9
12
12
12
12Running
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How to fill out sequencing with nextera rapid
How to fill out sequencing with nextera rapid
01
Start by preparing your DNA samples. Ensure that the samples are of high quality and have a concentration of at least 1 ng/μL.
02
Dilute the DNA samples to a desired concentration if necessary.
03
Set up the Nextera Rapid Capture Enzyme Mix and Tagmentation Reaction Mix according to the manufacturer's instructions.
04
Add the DNA samples to the Tagmentation Reaction Mix and mix gently.
05
Incubate the reaction mixture at 55°C for a specific duration, usually around 5 minutes.
06
Stop the Tagmentation Reaction by adding neutralization buffer and mix gently.
07
Prepare the library amplification reaction by adding the required components.
08
Amplify the library fragments using PCR amplification.
09
Check the library quality using DNA quantification methods.
10
Proceed with sequencing the libraries using a compatible sequencing platform following the manufacturer's instructions.
Who needs sequencing with nextera rapid?
01
Sequencing with Nextera Rapid is commonly used in various fields and applications, including but not limited to:
02
- Genomic research
03
- Transcriptomics and gene expression analysis
04
- Clinical diagnostics
05
- Microbial identification and characterization
06
- Evolutionary biology
07
- Forensic analysis
08
- Environmental studies
09
- Crop improvement and breeding
10
- Pharmacogenomics
11
Anyone seeking to analyze genetic information or study DNA samples can benefit from sequencing with Nextera Rapid.
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