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Establishing Tissue Culture of King of Bitters (Angiographic manipulate) and Comparison ofCallusVersus Plant Neoandrographolide Production by Cassandra Sialkot thesis presented to the Honors College
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01
Step 1: Prepare the necessary equipment and materials, including sterile growth medium, tissue culture vessels, scalpel or razor blade, and plant material.
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Step 2: Sterilize the plant material by washing it with a disinfectant solution and rinsing thoroughly with sterile water.
03
Step 3: Using a clean scalpel or razor blade, aseptically cut small sections of plant tissue, such as shoot tips or leaves.
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Step 4: Transfer the plant tissue onto the sterile growth medium in the tissue culture vessels.
05
Step 5: Incubate the vessels in a controlled environment with proper temperature, humidity, and lighting conditions.
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Step 6: Monitor the growth of the tissue culture regularly and provide necessary nutrients and hormones to support its development.
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Step 7: Once the tissue culture has developed into a plantlet, transfer it to a separate container with soil or a suitable growing medium for further growth and acclimatization.

Who needs establishing tissue culture of?

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Establishing tissue culture can be beneficial for a variety of individuals and organizations, including:
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- Conservationists and botanical gardens engaged in the preservation of endangered plant species.
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- Pharmaceutical and biotechnology companies involved in the production of medicinal plants.
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- Educational institutions and plant enthusiasts interested in learning and experimenting with plant tissue culture techniques.

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Establishing tissue culture of refers to the process of growing cells or tissues in a controlled environment outside of their natural environment.
Anyone who is conducting research or experiments involving tissue culture may be required to file establishing tissue culture of.
Establishing tissue culture of may require filling out a form provided by the relevant regulatory body or institution.
The purpose of establishing tissue culture of is to study and manipulate the growth and behavior of cells or tissues for various research or commercial purposes.
The information required to be reported on establishing tissue culture of may include the type of cells or tissues being cultured, the purpose of the culture, and any relevant procedures or protocols.
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