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BASIC IMMUNOHISTOCHEMISTRY (IOC) & FLUORESCENCE INS ITU HYBRIDIZATION (FISH) WORKSHOP: APPLICATION ON FORMALINFIXED PARAFFINEMBEDDED (FCPE) TISSUEOrganiser: Department of Pathology, Faculty of Medicine,
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01
Prepare the tissue samples by embedding them in paraffin blocks.
02
Cut thin sections from the paraffin blocks using a microtome.
03
Deparaffinize the sections by placing them in xylene or a similar solvent.
04
Rehydrate the sections by passing them through a series of alcohol washes with decreasing concentrations.
05
Block endogenous peroxidase activity by incubating the sections in hydrogen peroxide.
06
Wash the sections with a buffer solution to remove any residual chemicals.
07
Apply a primary antibody specific to the target protein of interest and incubate the sections overnight.
08
Wash the sections again with a buffer solution to remove any unbound primary antibody.
09
Apply a secondary antibody conjugated to a detection enzyme, such as horseradish peroxidase.
10
Incubate the sections with the secondary antibody to allow for binding to the primary antibody.
11
Wash the sections again to remove any unbound secondary antibody.
12
Apply a chromogenic substrate that will react with the enzyme conjugated to the secondary antibody.
13
Incubate the sections with the substrate, allowing for visualization of the target protein by color development.
14
Counterstain the sections with a dye, if desired, to provide contrast or additional information.
15
Mount coverslips on the sections using a mounting medium.
16
Observe and analyze the stained sections under a microscope.

Who needs basic immunohistochemistry ihc amp?

01
Basic immunohistochemistry (IHC) amp is needed by researchers, pathologists, and scientists in the field of biology and medicine.
02
It is commonly used in research studies to visualize and quantify the expression and localization of specific proteins in tissue samples.
03
Pathologists use IHC to aid in the diagnosis and classification of diseases, as certain proteins can be markers for different disease states.
04
Scientists may also use IHC to study protein expression patterns, understand cellular processes, and investigate the effects of various treatments or interventions.

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Basic immunohistochemistry ihc amp is a method used to detect specific proteins in tissue samples.
Researchers or healthcare professionals performing immunohistochemistry studies are required to file basic ihc amp forms.
Basic immunohistochemistry ihc amp forms can be filled out by providing details of the study, specimen, staining method, and results.
The purpose of basic immunohistochemistry ihc amp is to document the process and findings of immunohistochemistry studies.
Basic ihc amp forms must report details of the study protocol, specimen type, staining procedure, and interpretation of results.
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