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ES Cell Microinjection into Blastocysts Service FormBecause of the inherent biologic variability of ES cells, no guarantee is made for the number of chimeric mice produced, the extent of chimer ism,
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01
Prepare the ES cell microinjection setup by sterilizing all the equipment and workspace.
02
Choose the appropriate ES cell line and culture it to the desired stage.
03
Prepare the DNA construct or gene of interest for injection by purifying it and adjusting the concentration.
04
Load the DNA construct into a microinjection needle and adjust the injection pressure.
05
Anesthetize the recipient animal and carefully position it for injection.
06
Insert the microinjection needle into the target area, such as the blastocyst or pronucleus.
07
Inject the DNA construct into the target cells slowly and carefully.
08
Remove the microinjection needle and allow the injected cells to recover.
09
Transfer the injected cells or embryos into a suitable culture medium or recipient animal for further development.
10
Monitor the development of injected cells or embryos for successful integration and desired outcomes.

Who needs es cell microinjection into?

01
ES cell microinjection into is needed by researchers and scientists working in the field of developmental biology, genetic engineering, and stem cell research.
02
It is particularly useful for generating genetically modified organisms, studying gene function, and creating animal models of human diseases.
03
ES cell microinjection into is also utilized in various research projects aimed at understanding embryonic development and cellular differentiation.

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ES cell microinjection is into the blastocyst.
Researchers conducting genetic modification studies.
The process involves injecting embryonic stem cells into a blastocyst using a microinjection needle.
The purpose is to genetically modify the resulting organism.
Details of the genetic modification, methodology used, and any potential risks.
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