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BiologyName Per. Understanding Electrophoresis and PCR Restriction Digests of DNA Samples In 1968, Dr. Werner Amber at the University of Basel, Switzerland and Dr. Hamilton Smith at the Johns Hopkins
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01
Start by preparing a reaction mixture containing template DNA, primers, nucleotides, and DNA polymerase.
02
Denature the template DNA by heating it to a high temperature, usually around 95°C.
03
Once the template DNA is denatured, anneal the primers to the template DNA by cooling the reaction mixture.
04
Extend the primers by allowing DNA polymerase to add complementary nucleotides to the annealed primers, resulting in the synthesis of new DNA strands.
05
Repeat the denaturation, annealing, and extension steps for multiple cycles to amplify the DNA of interest.
06
Finally, analyze the amplified DNA using techniques such as gel electrophoresis or DNA sequencing.

Who needs use of polymerase chain?

01
Polymerase chain reaction (PCR) is widely used in various fields of research and applications:
02
- Molecular biology: PCR is used for amplifying specific DNA sequences for studies on gene expression, cloning, and sequencing.
03
- Diagnostic medicine: PCR is used for detecting and diagnosing various diseases, including infectious diseases, genetic disorders, and cancer.
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- Forensic science: PCR is used for DNA profiling and identifying individuals in criminal investigations.
05
- Food industry: PCR is used for detecting and quantifying genetically modified organisms (GMOs) in food products.
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- Environmental research: PCR is used for studying the biodiversity, population genetics, and microbial communities in different ecosystems.

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The use of polymerase chain is to amplify a specific segment of DNA.
Researchers or laboratories conducting PCR experiments are required to file use of polymerase chain.
Use of polymerase chain can be filled out by providing detailed information about the PCR experiment being conducted.
The purpose of use of polymerase chain is to monitor and regulate the use of PCR experiments to ensure safety and compliance.
Information such as the type of PCR experiment, target DNA segment, location of experiment, safety measures, and personnel involved must be reported on use of polymerase chain.
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