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1. Preparation of Blood Agar (BA)2. Objectives and scopes SOP describes the preparation of Blood Agar (BA) media that is used in the laboratory to determine or rule out contamination of positive MIT
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01
To prepare blood agar, follow these steps:
02
Prepare a nutrient agar base according to the manufacturer's instructions.
03
Autoclave the nutrient agar to sterilize it.
04
Allow the agar to cool down to around 45-50°C.
05
Aseptically add sterile defibrinated blood to the agar at a final concentration of 5-10%.
06
Mix the agar gently to ensure even distribution of the blood.
07
Pour the agar into Petri dishes.
08
Allow the agar to solidify at room temperature.
09
Once solid, store the blood agar plates upside down in a refrigerator until ready to use.

Who needs preparation of blood agar?

01
Preparation of blood agar is needed by microbiologists and laboratory technicians who perform microbiological culture and sensitivity testing.
02
Blood agar is commonly used to cultivate bacteria, particularly fastidious organisms that require additional nutrients and factors present in blood.
03
It is also used to detect hemolysis, which is the breakdown of red blood cells and can aid in the identification of certain bacteria species.

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Preparation of blood agar is the process of combining nutrient agar with blood to create a medium for the growth of bacteria.
Lab technicians or microbiologists are usually required to perform and document the preparation of blood agar.
Preparation of blood agar is typically filled out by recording the date, type of blood used, agar composition, and any additional ingredients or additives.
The purpose of preparation of blood agar is to provide a suitable environment for the cultivation and identification of bacteria present in a sample.
Information such as the date of preparation, source of blood, agar composition, and any additional additives should be reported on the preparation of blood agar form.
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