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Bacteriology Exercise NameObserving Bacterial Structure: Gram Stain To observe the structure of bacteria, we often use a process called the Gram stain developed in Europe by Hans Christian Gram (1884).

How to fill out observing bacterial structure gram

How to fill out observing bacterial structure gram

1. Prepare a bacterial smear slide by placing a small drop of bacteria culture in the center of a clean microscope slide.
2. Let the smear air-dry completely to ensure the bacteria adhere to the slide.
3. Fix the smear by passing the slide through a flame a few times or by adding a drop of methanol onto the slide.
4. Flood the smear with crystal violet stain and let it sit for 1 minute.
5. Gently rinse off the excess stain with water.
6. Flood the smear with iodine solution and let it sit for 1 minute. This helps to fix the crystal violet stain.
7. Gently rinse off the excess iodine with water.
8. Decolorize the smear by adding a few drops of 95% ethanol or acetone onto the slide and rinse it off immediately with water.
9. Counterstain the smear with safranin stain and let it sit for 1 minute.
10. Gently rinse off the excess safranin with water.
11. Blot dry the slide gently with a paper towel and observe the bacterial structure under a microscope.

Who needs observing bacterial structure gram?

1. Microbiologists
2. Medical professionals
3. Laboratory technicians
4. Researchers

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What is observing bacterial structure gram?

Observing bacterial structure gram is a laboratory technique used to differentiate bacteria based on their cell wall composition.

Who is required to file observing bacterial structure gram?

Lab technicians or scientists conducting bacterial analysis are required to file observing bacterial structure gram.

How to fill out observing bacterial structure gram?

To fill out observing bacterial structure gram, one must carefully observe the appearance of bacterial cells under a microscope and document the findings.

What is the purpose of observing bacterial structure gram?

The purpose of observing bacterial structure gram is to classify bacteria into two major groups: Gram-positive and Gram-negative, based on their response to staining.

What information must be reported on observing bacterial structure gram?

The information reported on observing bacterial structure gram includes the type of bacteria observed, their morphology, and whether they are Gram-positive or Gram-negative.