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Name Class Date Skills Practice Lab Gram Staining of Bacteria A stain gives color to cells that are colorless, such as bacterial cells. In 1884 the Danish physician Hans Christian Gram developed the
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How to fill out gram staining of bacteria

01
Prepare a thin bacterial smear by spreading a small amount of the bacterial culture onto a clean glass slide.
02
Allow the smear to air dry completely.
03
Fix the smear by passing the slide through a flame a few times. Be careful not to overheat and distort the bacteria.
04
Flood the smear with crystal violet stain and let it sit for 1 minute.
05
Rinse the slide with water to remove excess stain.
06
Flood the smear with iodine solution and let it sit for 1 minute. The iodine acts as a mordant to intensify the staining.
07
Rinse the slide with water to remove excess iodine.
08
Decolorize the smear by pouring alcohol or acetone over the slide until no more color runs off.
09
Immediately rinse the slide with water to stop the decolorization.
10
Counterstain the smear with safranin stain and let it sit for 1 minute.
11
Rinse the slide with water to remove excess stain.
12
Gently blot the slide to remove excess water and air dry it completely before examining under a microscope.

Who needs gram staining of bacteria?

01
Gram staining of bacteria is needed by microbiologists, researchers, and medical professionals to identify and classify bacteria based on their cell wall characteristics.
02
It is an essential technique used in the field of microbiology to differentiate bacteria into Gram-positive (retaining violet stain) and Gram-negative (retaining safranin counterstain) based on their reaction to the staining process.
03
This information helps in diagnosing and treating bacterial infections, identifying bacterial species, and determining appropriate antibiotic treatments.

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