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PHOTOSYNTHETIC PIGMENTS LAB PAPER CHROMATOGRAPHYPhotosynthetic Pigments: Background The photosynthetic pigments are responsible for absorbing and trapping light energy in the early steps of photosynthesis.
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How to fill out photosynformtic pigments lab
How to fill out photosynformtic pigments lab
01
Start by gathering all the necessary materials for the photosynthetic pigments lab, including plant leaves, acetone, mortar and pestle, filter paper, chromatography paper, and a developing solvent like petroleum ether.
02
Begin by grinding the plant leaves in a mortar and pestle until they become a homogenous green paste.
03
Transfer the ground leaves into a test tube and add a small amount of acetone. Mix well to extract the pigments from the plant material.
04
Place a piece of filter paper into the test tube and gently shake it to allow the pigments to dissolve into the acetone.
05
Carefully remove the filter paper from the test tube and allow it to dry completely.
06
Cut a small strip of chromatography paper and draw a horizontal line near one end using a pencil.
07
Using a capillary tube, apply a small spot of the pigment extract onto the drawn line on the chromatography paper.
08
Prepare a developing solvent by mixing petroleum ether with a small amount of acetone. Pour the solvent into a developing chamber, ensuring it is deep enough to immerse the chromatography paper partially.
09
Hang the chromatography paper vertically in the chamber, making sure the spot does not touch the solvent.
10
Cover the chamber with a lid and allow the solvent to rise up the paper, separating the different pigments present in the extract.
11
Once the solvent front reaches close to the top of the paper, remove it from the chamber and mark the solvent front with a pencil.
12
Leave the chromatography paper to dry and observe the separated pigments, noting their respective Rf values for analysis.
Who needs photosynformtic pigments lab?
01
Photosynthetic pigments labs are valuable for biology students, researchers, and scientists studying plant physiology and photosynthesis.
02
These labs help in understanding the various pigments involved in capturing light energy during photosynthesis, such as chlorophylls, carotenoids, and phycobilins.
03
They can also be useful for botanists, agronomists, and environmental scientists interested in studying plant pigments and their ecological significance.
04
Furthermore, these labs are beneficial in educational settings to teach students about the principles of photosynthesis and the role of pigments in energy conversion.
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What is photosynformtic pigments lab?
Photosynformtic pigments lab is a laboratory procedure used to analyze the pigments involved in the process of photosynthesis.
Who is required to file photosynformtic pigments lab?
Scientists, researchers, and students studying photosynthesis may be required to conduct and file a photosynformtic pigments lab.
How to fill out photosynformtic pigments lab?
To fill out a photosynformtic pigments lab, one must follow the specific protocol provided by the laboratory or academic institution.
What is the purpose of photosynformtic pigments lab?
The purpose of a photosynformtic pigments lab is to understand the types and quantities of pigments present in plant cells involved in photosynthesis.
What information must be reported on photosynformtic pigments lab?
The information reported on a photosynformtic pigments lab may include types of pigments, their concentrations, and any experimental findings.
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