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Anti CCP ELISA GWB521226 www.genwaybio.com FOR RESEARCH USE Holoenzyme immunoassay for the quantitative determination of anti CCP in human serum or plasma1. INTRODUCTION Rheumatoid arthritis (RA)
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01
Start by preparing the laboratory equipment and reagents needed for the Anti-CCP ELISA test.
02
Label the wells on the ELISA plate according to the samples you have.
03
Add the positive control, negative control, and patient samples to the respective wells in duplicate.
04
Incubate the plate at the recommended temperature and time to allow the binding of Anti-CCP antibodies.
05
Wash the wells to remove unbound substances and add the enzyme conjugate.
06
Incubate the plate again to allow the binding of the enzyme conjugate to the Anti-CCP antibodies.
07
Wash the wells again and add the substrate solution.
08
Incubate the plate to allow the substrate to react with the enzyme conjugate, leading to a color change.
09
Stop the reaction by adding a stop solution.
10
Measure the absorbance of each well using a spectrophotometer and record the results.
11
Analyze the results to determine the presence and levels of Anti-CCP antibodies in the samples.

Who needs anti ccp elisa?

01
Anti-CCP ELISA is commonly used to diagnose and monitor rheumatoid arthritis (RA).
02
It is especially useful for confirming the presence of anti-cyclic citrullinated peptide (anti-CCP) antibodies in the blood, which are highly specific to RA.
03
Therefore, individuals suspected or diagnosed with rheumatoid arthritis may need to undergo an Anti-CCP ELISA test.
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Anti CCP ELISA is a blood test used to detect antibodies against cyclic citrullinated peptide in the body. It is commonly used to diagnose and monitor rheumatoid arthritis.
Patients who are suspected to have rheumatoid arthritis may be required to undergo an anti CCP ELISA test.
The anti CCP ELISA test requires a blood sample to be collected from the patient and sent to a laboratory for analysis.
The purpose of anti CCP ELISA is to help diagnose rheumatoid arthritis and monitor disease progression.
The results of the anti CCP ELISA test, including the levels of antibodies against cyclic citrullinated peptide, must be reported.
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