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Next Generation Sequencing in
the New York State Newborn
Screening Molecular Lab
Colleen Stevens, Ph.D.
Research Scientist
NYS Newborn Screening Program
February 22, 2017February 22, 20172NYS CF Newborn
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How to fill out next generation sequencing in
How to fill out next generation sequencing in
01
Start by preparing your DNA or RNA samples to be sequenced.
02
Obtain a next generation sequencing platform, such as Illumina or Ion Torrent.
03
Set up the sequencing instrument according to the manufacturer's instructions.
04
Prepare the sequencing library by fragmenting the DNA or RNA, adding adapters, and amplifying the fragments.
05
Load the library onto the sequencing instrument and start the sequencing run.
06
Monitor the sequencing run to ensure proper data generation and quality control.
07
Once the run is complete, analyze the raw sequencing data to obtain the sequences of interest.
08
Perform bioinformatics analysis to align the sequences, detect variants, or perform other desired analyses.
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Interpret the results and draw relevant conclusions based on the sequencing data obtained.
10
Document and report the findings of the next generation sequencing experiment.
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01
Researchers in the field of genomics and genetics who want to study the DNA or RNA sequences of organisms.
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What is next generation sequencing in?
Next generation sequencing is a technology that allows for rapid sequencing of DNA and RNA.
Who is required to file next generation sequencing in?
Researchers, scientists, and laboratories are required to file next generation sequencing in.
How to fill out next generation sequencing in?
Next generation sequencing can be filled out by inputting the sequencing data into a specialized software program.
What is the purpose of next generation sequencing in?
The purpose of next generation sequencing is to analyze genetic variations, study gene expression, and uncover disease mechanisms.
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Information such as sequencing data, sample information, and experimental conditions must be reported on next generation sequencing in.
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