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Journal of Visualized Experiments. Jove.materials List for:Mouse Sperm Cryopreservation and Recovery using the Cry Kit
111Ling Liu, Steven R. Sensing, Iva S. Morse, Kathleen R. PritchettCorning21Genetically
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How to fill out mouse sperm cryopreservation and

How to fill out mouse sperm cryopreservation and
01
To fill out mouse sperm cryopreservation, follow these steps:
02
Prepare the cryopreservation medium according to the manufacturer's instructions.
03
Collect the male mice and isolate their testes.
04
Remove the testes from the mice and place them in a sterile container.
05
Gently mince the testes with a pair of scissors to release the sperm.
06
Transfer the minced testes into a centrifuge tube and centrifuge at a low speed to separate the sperm from the tissue fragments.
07
Carefully remove the supernatant using a pipette and transfer it into a new centrifuge tube.
08
Add the cryopreservation medium to the tube containing the sperm, following the recommended ratio.
09
Mix the sperm and cryopreservation medium gently by pipetting up and down.
10
Label the tube with the date and mouse strain information.
11
Place the tube in a freezing container and gradually cool it down using a controlled freezing device.
12
Once the temperature reaches around -80 degrees Celsius, transfer the tube to a storage container filled with liquid nitrogen for long-term storage.
13
Remember to follow proper safety protocols and sterile techniques throughout the entire process.
Who needs mouse sperm cryopreservation and?
01
Mouse sperm cryopreservation is needed by:
02
- Researchers and scientists working with genetically modified mouse models, as a way to preserve valuable mouse strains for future use.
03
- Biotechnology companies that specialize in the production and distribution of mouse strains for research purposes.
04
- Mouse breeding facilities and repositories that aim to maintain a diverse collection of mouse strains for scientific studies.
05
Overall, mouse sperm cryopreservation is essential for preserving mouse genetic material and ensuring the availability of specific mouse strains for various research purposes.
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