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Blood BankNameAntiHuman Globulin (AHG)General Information
In the past, AHG (C bomb\'s era) was obtained from rabbits immunized to human GAM my globulin.
The resulting antis era would react with human
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How to fill out anti-human globulin ahg
How to fill out anti-human globulin (AHG):
01
Begin by checking the expiration date on the AHG reagent. Make sure it is within the valid range.
02
Prepare a clean and sterile work area. Use gloves to minimize contamination.
03
Gently mix the AHG reagent by inverting the vial several times to ensure the components are well-mixed.
04
Take a small volume of patient's washed red blood cells (RBCs) in a clean test tube. The specific volume may vary depending on the test being performed, so refer to the protocol or manufacturer's instructions.
05
Add the desired volume of AHG reagent to the test tube containing the RBCs. Again, the recommended volume may vary, so follow the instructions carefully.
06
Mix the contents of the test tube thoroughly by gently inverting it several times. Avoid vigorous shaking, as this may cause RBC agglutination.
07
Incubate the test tube at the appropriate temperature and duration specified in the test protocol. Usually, the recommended incubation time is around 15-30 minutes at 37°C.
08
After incubation, gently centrifuge the test tube at a low speed to pellet the sensitized RBCs.
09
Carefully decant or aspirate the supernatant, being cautious not to disturb the pellet.
10
Resuspend the RBC pellet with an appropriate volume of saline or buffer solution, depending on the subsequent test or procedure.
11
The AHG-treated RBCs are now ready for further testing or analysis, as required.
Who needs anti-human globulin (AHG)?
01
AHG is used in various clinical scenarios, particularly in the field of blood banking and immunohematology.
02
Typically, AHG is utilized in compatibility testing for blood transfusion purposes. It helps detect immune-mediated RBC sensitization and aids in identifying potential transfusion reactions.
03
It is also employed in detecting antibodies that may cause autoimmune hemolytic anemia or certain types of hemolytic disease of the fetus and newborn.
04
AHG testing may be necessary for patients undergoing prenatal testing, autoimmune disease diagnosis, or investigations related to certain blood disorders.
05
The decision to use AHG is made based on the clinical indication and specific guidelines provided by the laboratory or healthcare provider.
06
Only qualified medical professionals, such as laboratory technologists or pathologists, should perform and interpret AHG testing.
Please note that the specific instructions, volumes, and protocols for using AHG may vary depending on the test being conducted. It is essential to follow the manufacturer's instructions and consult relevant laboratory protocols for accurate and reliable results.
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What is anti-human globulin ahg?
Anti-human globulin (AHG) is a reagent used in blood banking to detect the presence of antibodies on the surface of red blood cells.
Who is required to file anti-human globulin ahg?
Healthcare professionals in blood banking and transfusion medicine are required to perform and interpret anti-human globulin (AHG) testing.
How to fill out anti-human globulin ahg?
Anti-human globulin (AHG) testing is typically performed in a laboratory setting following established protocols and procedures.
What is the purpose of anti-human globulin ahg?
The purpose of anti-human globulin (AHG) testing is to detect the presence of antibodies that could cause adverse reactions during blood transfusions.
What information must be reported on anti-human globulin ahg?
The results of the anti-human globulin (AHG) test, including the presence or absence of agglutination, must be reported.
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